Protein synthesis and axonal transport during nerve regeneration

G. W. Perry, D. L. Wilson

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Protein synthesis and axonal transport have been studied in regenerating peripheral nerves. Sciatic nerves of bullfrogs were unilaterally crushed or cut. The animals were killed 1, 2, or 4 weeks later, and 8th and 9th dorsal root ganglia removed together with sciatic nerves and dorsal roots. The ganglia were selectively labeled in vitro with [35S]-methionine. Labeled proteins, in dorsal root ganglia and rapidly transported to ligatures placed on the sciatic nerves and dorsal roots, were analyzed by two-dimensional polyacrylamide gel electrophoresis. Qualitative analysis of protein patterns revealed no totally new proteins synthesized or rapidly transported in regenerating nerves. However, quantitative comparison of regenerating and contralateral control nerves revealed significant differences in abundance for some of the proteins synthesized in dorsal root ganglia, and for a few of the rapidly transported proteins. Quantitative analysis of rapidly transported proteins in both the peripheral processes (spinal nerves) and central processes (dorsal roots) revealed similar changes despite the fact that the roots were undamaged. The overall lack of drastic changes seen in protein synthesis and transport suggests that the neuron in its program of normal maintenance synthesizes and supplies most of the materials required for axon regrowth.

Original languageEnglish
Pages (from-to)1203-1217
Number of pages15
JournalJournal of Neurochemistry
Volume37
Issue number5
StatePublished - Dec 1 1981

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Nerve Regeneration
Axonal Transport
Protein Transport
Spinal Nerve Roots
Spinal Ganglia
Sciatic Nerve
Proteins
Rana catesbeiana
Spinal Nerves
Electrophoresis, Gel, Two-Dimensional
Peripheral Nerves
Ganglia
Methionine
Ligation
Axons
Electrophoresis
Maintenance
Neurons
Animals
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Perry, G. W., & Wilson, D. L. (1981). Protein synthesis and axonal transport during nerve regeneration. Journal of Neurochemistry, 37(5), 1203-1217.

Protein synthesis and axonal transport during nerve regeneration. / Perry, G. W.; Wilson, D. L.

In: Journal of Neurochemistry, Vol. 37, No. 5, 01.12.1981, p. 1203-1217.

Research output: Contribution to journalArticle

Perry, GW & Wilson, DL 1981, 'Protein synthesis and axonal transport during nerve regeneration', Journal of Neurochemistry, vol. 37, no. 5, pp. 1203-1217.
Perry, G. W. ; Wilson, D. L. / Protein synthesis and axonal transport during nerve regeneration. In: Journal of Neurochemistry. 1981 ; Vol. 37, No. 5. pp. 1203-1217.
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