Protein precipitation assay for quantitation of tannins: Determination of protein in tannin-protein complex

Harinder Paul S. Makkar, Rajinder Dawra, Bhupinder Singh

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

A protein precipitation method for the determination of tannins has been developed. The protein in the tannin-protein complexes was measured using the ninhydrin assay of amino acids released by alkaline hydrolysis of the complex. Standard protein and the complex were hydrolyzed with 13.5 n NaOH at 120°C for 20 min and the amino acids released were measured with ninhydrin. Tannins did not interfere in the determination of protein by ninhydrin assay. The bovine serum albumin (BSA) precipitated (y;mg) increased linearly with increase in tannic acid (x) from 0.2 to 0.9 mg (y = 2.598x - 0.258). The protein precipitation capacities (mg BSA precipitated/g dry wt) measured by the method for young and mature leaves of oaks were Quercus incana (young, 42.21; mature, 79.51), Q. ilex (young, 1.86; mature, 1.86), and Q. semecarpifolia (young, 733.54; mature, 304.32). The method can provide valuable information on the mechanisms of protein-tannin interactions and nutritional and physiological significances of tannins.

Original languageEnglish (US)
Pages (from-to)435-439
Number of pages5
JournalAnalytical Biochemistry
Volume166
Issue number2
DOIs
StatePublished - Nov 1 1987
Externally publishedYes

Fingerprint

Tannins
Assays
Ninhydrin
Proteins
Bovine Serum Albumin
Ilex
Quercus
Amino Acids
Hydrolysis

Keywords

  • ninhydrin
  • protein-tannin complex
  • tannin determination

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Protein precipitation assay for quantitation of tannins : Determination of protein in tannin-protein complex. / Makkar, Harinder Paul S.; Dawra, Rajinder; Singh, Bhupinder.

In: Analytical Biochemistry, Vol. 166, No. 2, 01.11.1987, p. 435-439.

Research output: Contribution to journalArticle

@article{e24f9e5660c74da7a2115d3cafefd52b,
title = "Protein precipitation assay for quantitation of tannins: Determination of protein in tannin-protein complex",
abstract = "A protein precipitation method for the determination of tannins has been developed. The protein in the tannin-protein complexes was measured using the ninhydrin assay of amino acids released by alkaline hydrolysis of the complex. Standard protein and the complex were hydrolyzed with 13.5 n NaOH at 120°C for 20 min and the amino acids released were measured with ninhydrin. Tannins did not interfere in the determination of protein by ninhydrin assay. The bovine serum albumin (BSA) precipitated (y;mg) increased linearly with increase in tannic acid (x) from 0.2 to 0.9 mg (y = 2.598x - 0.258). The protein precipitation capacities (mg BSA precipitated/g dry wt) measured by the method for young and mature leaves of oaks were Quercus incana (young, 42.21; mature, 79.51), Q. ilex (young, 1.86; mature, 1.86), and Q. semecarpifolia (young, 733.54; mature, 304.32). The method can provide valuable information on the mechanisms of protein-tannin interactions and nutritional and physiological significances of tannins.",
keywords = "ninhydrin, protein-tannin complex, tannin determination",
author = "Makkar, {Harinder Paul S.} and Rajinder Dawra and Bhupinder Singh",
year = "1987",
month = "11",
day = "1",
doi = "10.1016/0003-2697(87)90596-3",
language = "English (US)",
volume = "166",
pages = "435--439",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Protein precipitation assay for quantitation of tannins

T2 - Determination of protein in tannin-protein complex

AU - Makkar, Harinder Paul S.

AU - Dawra, Rajinder

AU - Singh, Bhupinder

PY - 1987/11/1

Y1 - 1987/11/1

N2 - A protein precipitation method for the determination of tannins has been developed. The protein in the tannin-protein complexes was measured using the ninhydrin assay of amino acids released by alkaline hydrolysis of the complex. Standard protein and the complex were hydrolyzed with 13.5 n NaOH at 120°C for 20 min and the amino acids released were measured with ninhydrin. Tannins did not interfere in the determination of protein by ninhydrin assay. The bovine serum albumin (BSA) precipitated (y;mg) increased linearly with increase in tannic acid (x) from 0.2 to 0.9 mg (y = 2.598x - 0.258). The protein precipitation capacities (mg BSA precipitated/g dry wt) measured by the method for young and mature leaves of oaks were Quercus incana (young, 42.21; mature, 79.51), Q. ilex (young, 1.86; mature, 1.86), and Q. semecarpifolia (young, 733.54; mature, 304.32). The method can provide valuable information on the mechanisms of protein-tannin interactions and nutritional and physiological significances of tannins.

AB - A protein precipitation method for the determination of tannins has been developed. The protein in the tannin-protein complexes was measured using the ninhydrin assay of amino acids released by alkaline hydrolysis of the complex. Standard protein and the complex were hydrolyzed with 13.5 n NaOH at 120°C for 20 min and the amino acids released were measured with ninhydrin. Tannins did not interfere in the determination of protein by ninhydrin assay. The bovine serum albumin (BSA) precipitated (y;mg) increased linearly with increase in tannic acid (x) from 0.2 to 0.9 mg (y = 2.598x - 0.258). The protein precipitation capacities (mg BSA precipitated/g dry wt) measured by the method for young and mature leaves of oaks were Quercus incana (young, 42.21; mature, 79.51), Q. ilex (young, 1.86; mature, 1.86), and Q. semecarpifolia (young, 733.54; mature, 304.32). The method can provide valuable information on the mechanisms of protein-tannin interactions and nutritional and physiological significances of tannins.

KW - ninhydrin

KW - protein-tannin complex

KW - tannin determination

UR - http://www.scopus.com/inward/record.url?scp=0023441294&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023441294&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(87)90596-3

DO - 10.1016/0003-2697(87)90596-3

M3 - Article

C2 - 3434784

AN - SCOPUS:0023441294

VL - 166

SP - 435

EP - 439

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -