Protein phosphatase-1 binding to Scd5p is important for regulation of actin organization and endocytosis in yeast

Ji Suk Chang, Kenneth Henry, Bianka L. Wolf, Maribel Geli, Sandra Lemmon

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

SCD5, an essential gene, encodes a protein important for endocytosis and actin organization in yeast. Previous two-hybrid screens showed that Scd5p interacts with Glc7p, a yeast Ser/Thr-specific protein phosphatase-1 (PP1) that participates in a variety of cellular processes. PPI substrate specificity in vivo is regulated by association with different regulatory or targeting subunits, many of which have a consensus PPI -binding site ((V/I)XF, with a basic residue at the -1 or -2 position). Scd5p contains two of these potential PPI-binding motifs: KVDF (amino acids 240-243) and KKVRF (amino acids 272-276). Deletion analysis mapped the PPI-binding domain to a region of Scd5p containing these motifs. Therefore, the consequence of mutating these two potential PPI-binding sites was examined. Although mutation of KVDF had no effect, alteration of KKVRF dramatically reduced Scd5p interaction with Glc7p and resulted in temperature-sensitive growth. Furthermore, this mutation caused defects in fluid phase and receptor-mediated endocytosis and actin organization. Overexpression of GLC7 suppressed the temperature-sensitive growth of the KKVRF mutant and partially rescued the actin organization phenotype. These results provide evidence that Sed5p is a PPI targeting subunit for regulation of actin organization and endocytosis or that Scd5p is a PPI substrate, which regulates the function of Scd5p in these processes.

Original languageEnglish
Pages (from-to)48002-48008
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number50
DOIs
StatePublished - Dec 13 2002
Externally publishedYes

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Protein Phosphatase 1
Endocytosis
Yeast
Actins
Yeasts
Binding Sites
Amino Acids
Amino Acid Motifs
Mutation
Temperature
Essential Genes
Substrates
Growth
Substrate Specificity
Genes
Phenotype
Defects
Fluids
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Protein phosphatase-1 binding to Scd5p is important for regulation of actin organization and endocytosis in yeast. / Chang, Ji Suk; Henry, Kenneth; Wolf, Bianka L.; Geli, Maribel; Lemmon, Sandra.

In: Journal of Biological Chemistry, Vol. 277, No. 50, 13.12.2002, p. 48002-48008.

Research output: Contribution to journalArticle

Chang, Ji Suk ; Henry, Kenneth ; Wolf, Bianka L. ; Geli, Maribel ; Lemmon, Sandra. / Protein phosphatase-1 binding to Scd5p is important for regulation of actin organization and endocytosis in yeast. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 50. pp. 48002-48008.
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