Protein nitration, metabolites of reactive nitrogen species, and inflammation in lung allografts

João A. De Andrade, John P. Crow, Liliana Viera, C. Bruce Alexander, K. Randall Young, David C. McGiffin, George L. Zorn, Sha Zhu, Sadis Matalon, Robert Jackson

Research output: Contribution to journalArticle

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Abstract

This study investigated nitration and chlorination of epithelial lining fluid (ELF) proteins in patients (n = 29) who had undergone lung allotransplantation. We assayed lung lavage nitrotyrosine (NT) and chlorotyrosine (CT) by HPLC. We measured NT, nitrate (NO3-), and nitrate (NO2-) in bronchoalveolar lavage fluid (BALF) and total nitrite (NO2- + NO3-) in serum of another group of lung transplant patients (n = 82). In the first group (n = 29), percent nitration of tyrosines (Tyr) (NT/total Tyr x 100) in BALF proteins was: patients, 0.01 (0.00-0.12)%; median (25th-75th% confidence interval), and control subjects 0.01 (0.00-0.02)%. CT (CT/total Tyr x 100) occurred only in the patients' BALF: 0.01 (0.00-0.02)%. In the second group (n = 82), nitrotyrosine (NT) was detected by ELISA in the BALF of patients: 9 (0-41) pmol/mg pro and control subjects: 28 (26-33). Total nitrite (NO2- + NO3-) in BALF of the patients: 3.3 (1.9-5.1) μM significantly exceeded that in control subjects: 1.3 (0.8-1.3) μM; p = 0.0133. Serum nitrite also was significantly higher in patients: 37 (26-55) μM than control subjects: 19 (17-20) μM; p = 0.0037. Airway inflammation in transbronchial biopsies (B score) correlated with NT in BALF (p = 0.0369). Lung transplants have increased airway concentrations of reactive nitrogen species (RNS) metabolites. NT, a marker of peroxynitrite (ONOO-), is related to the degree of airway inflammation in lung transplants.

Original languageEnglish
Pages (from-to)2035-2042
Number of pages8
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume161
Issue number6
StatePublished - Jul 11 2000
Externally publishedYes

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Reactive Nitrogen Species
Bronchoalveolar Lavage Fluid
Allografts
Pneumonia
Nitrites
Proteins
Tyrosine
Transplants
Nitrates
Lung
Peroxynitrous Acid
Halogenation
Bronchoalveolar Lavage
Serum
3-nitrotyrosine
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography
Confidence Intervals
Inflammation
Biopsy

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

De Andrade, J. A., Crow, J. P., Viera, L., Alexander, C. B., Young, K. R., McGiffin, D. C., ... Jackson, R. (2000). Protein nitration, metabolites of reactive nitrogen species, and inflammation in lung allografts. American Journal of Respiratory and Critical Care Medicine, 161(6), 2035-2042.

Protein nitration, metabolites of reactive nitrogen species, and inflammation in lung allografts. / De Andrade, João A.; Crow, John P.; Viera, Liliana; Alexander, C. Bruce; Young, K. Randall; McGiffin, David C.; Zorn, George L.; Zhu, Sha; Matalon, Sadis; Jackson, Robert.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 161, No. 6, 11.07.2000, p. 2035-2042.

Research output: Contribution to journalArticle

De Andrade, JA, Crow, JP, Viera, L, Alexander, CB, Young, KR, McGiffin, DC, Zorn, GL, Zhu, S, Matalon, S & Jackson, R 2000, 'Protein nitration, metabolites of reactive nitrogen species, and inflammation in lung allografts', American Journal of Respiratory and Critical Care Medicine, vol. 161, no. 6, pp. 2035-2042.
De Andrade JA, Crow JP, Viera L, Alexander CB, Young KR, McGiffin DC et al. Protein nitration, metabolites of reactive nitrogen species, and inflammation in lung allografts. American Journal of Respiratory and Critical Care Medicine. 2000 Jul 11;161(6):2035-2042.
De Andrade, João A. ; Crow, John P. ; Viera, Liliana ; Alexander, C. Bruce ; Young, K. Randall ; McGiffin, David C. ; Zorn, George L. ; Zhu, Sha ; Matalon, Sadis ; Jackson, Robert. / Protein nitration, metabolites of reactive nitrogen species, and inflammation in lung allografts. In: American Journal of Respiratory and Critical Care Medicine. 2000 ; Vol. 161, No. 6. pp. 2035-2042.
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AU - Young, K. Randall

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