Protein kinase regulation of tumor necrosis factor alpha stimulated collagenase and stromelysin message levels in chondrocytes

P. G. Mitchell, Herman S Cheung

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

TNF stimulated transcription and secretion of the metalloproteinases collagenase and stromelysin in porcine articular chondrocytes. TNF induced metalloproteinase transcription could be inhibited with either protein kinase inhibitors (H7 or staurosporine) or by raising intracellular cAMP levels. HA1004, a protein kinase inhibitor structurally related to H7 but with a higher Ki for protein kinase C had no effect on TNF induced message levels. TNF treatment of chondrocytes did not induce membrane associated PKC or increase intracellular cAMP levels. Our results are consistent with the involvement of a staurosporine and H7 sensitive protein kinase distinct from PKC in TNF signal transduction in chondrocytes.

Original languageEnglish
Pages (from-to)1133-1142
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume196
Issue number3
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 3
Staurosporine
Metalloproteases
Collagenases
Transcription
Protein Kinase Inhibitors
Chondrocytes
Protein Kinases
Tumor Necrosis Factor-alpha
Signal transduction
Protein Kinase C
Membranes
Signal Transduction
Swine
Joints

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

@article{8564d48e37844558bca81a86904360b6,
title = "Protein kinase regulation of tumor necrosis factor alpha stimulated collagenase and stromelysin message levels in chondrocytes",
abstract = "TNF stimulated transcription and secretion of the metalloproteinases collagenase and stromelysin in porcine articular chondrocytes. TNF induced metalloproteinase transcription could be inhibited with either protein kinase inhibitors (H7 or staurosporine) or by raising intracellular cAMP levels. HA1004, a protein kinase inhibitor structurally related to H7 but with a higher Ki for protein kinase C had no effect on TNF induced message levels. TNF treatment of chondrocytes did not induce membrane associated PKC or increase intracellular cAMP levels. Our results are consistent with the involvement of a staurosporine and H7 sensitive protein kinase distinct from PKC in TNF signal transduction in chondrocytes.",
author = "Mitchell, {P. G.} and Cheung, {Herman S}",
year = "1993",
month = "1",
day = "1",
doi = "10.1006/bbrc.1993.2369",
language = "English",
volume = "196",
pages = "1133--1142",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Protein kinase regulation of tumor necrosis factor alpha stimulated collagenase and stromelysin message levels in chondrocytes

AU - Mitchell, P. G.

AU - Cheung, Herman S

PY - 1993/1/1

Y1 - 1993/1/1

N2 - TNF stimulated transcription and secretion of the metalloproteinases collagenase and stromelysin in porcine articular chondrocytes. TNF induced metalloproteinase transcription could be inhibited with either protein kinase inhibitors (H7 or staurosporine) or by raising intracellular cAMP levels. HA1004, a protein kinase inhibitor structurally related to H7 but with a higher Ki for protein kinase C had no effect on TNF induced message levels. TNF treatment of chondrocytes did not induce membrane associated PKC or increase intracellular cAMP levels. Our results are consistent with the involvement of a staurosporine and H7 sensitive protein kinase distinct from PKC in TNF signal transduction in chondrocytes.

AB - TNF stimulated transcription and secretion of the metalloproteinases collagenase and stromelysin in porcine articular chondrocytes. TNF induced metalloproteinase transcription could be inhibited with either protein kinase inhibitors (H7 or staurosporine) or by raising intracellular cAMP levels. HA1004, a protein kinase inhibitor structurally related to H7 but with a higher Ki for protein kinase C had no effect on TNF induced message levels. TNF treatment of chondrocytes did not induce membrane associated PKC or increase intracellular cAMP levels. Our results are consistent with the involvement of a staurosporine and H7 sensitive protein kinase distinct from PKC in TNF signal transduction in chondrocytes.

UR - http://www.scopus.com/inward/record.url?scp=0027491431&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027491431&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1993.2369

DO - 10.1006/bbrc.1993.2369

M3 - Article

C2 - 7504465

AN - SCOPUS:0027491431

VL - 196

SP - 1133

EP - 1142

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -