Abstract
TNF stimulated transcription and secretion of the metalloproteinases collagenase and stromelysin in porcine articular chondrocytes. TNF induced metalloproteinase transcription could be inhibited with either protein kinase inhibitors (H7 or staurosporine) or by raising intracellular cAMP levels. HA1004, a protein kinase inhibitor structurally related to H7 but with a higher Ki for protein kinase C had no effect on TNF induced message levels. TNF treatment of chondrocytes did not induce membrane associated PKC or increase intracellular cAMP levels. Our results are consistent with the involvement of a staurosporine and H7 sensitive protein kinase distinct from PKC in TNF signal transduction in chondrocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 1133-1142 |
| Number of pages | 10 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 196 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Protein kinase regulation of tumor necrosis factor alpha stimulated collagenase and stromelysin message levels in chondrocytes. / Mitchell, P. G.; Cheung, Herman S.
In: Biochemical and Biophysical Research Communications, Vol. 196, No. 3, 01.01.1993, p. 1133-1142.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protein kinase regulation of tumor necrosis factor alpha stimulated collagenase and stromelysin message levels in chondrocytes
AU - Mitchell, P. G.
AU - Cheung, Herman S
PY - 1993/1/1
Y1 - 1993/1/1
N2 - TNF stimulated transcription and secretion of the metalloproteinases collagenase and stromelysin in porcine articular chondrocytes. TNF induced metalloproteinase transcription could be inhibited with either protein kinase inhibitors (H7 or staurosporine) or by raising intracellular cAMP levels. HA1004, a protein kinase inhibitor structurally related to H7 but with a higher Ki for protein kinase C had no effect on TNF induced message levels. TNF treatment of chondrocytes did not induce membrane associated PKC or increase intracellular cAMP levels. Our results are consistent with the involvement of a staurosporine and H7 sensitive protein kinase distinct from PKC in TNF signal transduction in chondrocytes.
AB - TNF stimulated transcription and secretion of the metalloproteinases collagenase and stromelysin in porcine articular chondrocytes. TNF induced metalloproteinase transcription could be inhibited with either protein kinase inhibitors (H7 or staurosporine) or by raising intracellular cAMP levels. HA1004, a protein kinase inhibitor structurally related to H7 but with a higher Ki for protein kinase C had no effect on TNF induced message levels. TNF treatment of chondrocytes did not induce membrane associated PKC or increase intracellular cAMP levels. Our results are consistent with the involvement of a staurosporine and H7 sensitive protein kinase distinct from PKC in TNF signal transduction in chondrocytes.
UR - http://www.scopus.com/inward/record.url?scp=0027491431&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027491431&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1993.2369
DO - 10.1006/bbrc.1993.2369
M3 - Article
C2 - 7504465
AN - SCOPUS:0027491431
VL - 196
SP - 1133
EP - 1142
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -