Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP

Silvia Moreno, Ricardo Pastori, Susana Passeron

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Cyclic AMP binding to Mucor rouxii protein kinase holoenzyme and free regulatory subunit was measured by the classical membrane filtration technique and by equilibrium dialysis. The results obtained demonstrate that the filtration method can be used without loss of any cyclic AMP binding site. Both methods unambiguously demonstrate that the number of molecules of cyclic AMP bound to the holoenzyme are half of those bound to the regulatory subunit. This result suggests that unshielding of new cyclic AMP binding sites occurs upon dissociation of the ternary complex holoenzyme-cyclic AMP.

Original languageEnglish
Pages (from-to)13-16
Number of pages4
JournalMolecular and Cellular Biochemistry
Volume52
Issue number1
DOIs
StatePublished - Mar 1 1983
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

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