Protein kinase C activity affects glucose-induced oscillations in cytoplasmic free Ca2+ in the pancreatic B-cell

Henrik Kindmark, Martin Köhler, Suad Efendić, Patrik Rorsman, Olof Larsson, Per Olof Berggren

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Acute stimulation of protein kinase C (PKC) inhibited glucose-induced slow oscillations in cytoplasmic free Ca2+-concentration, [Ca2+]i, in mouse pancreatic B-cells. In PKC-depleted cells glucose induced rapid transients in [Ca2+]i, lasting for approximately 10 s, superimposed on the slow oscillations in [Ca2+]i. It was demonstrated that the transients did not occur in the absence of extracellular Ca2+. Each transient typically was preceded by a slow increase in [Ca2+]i, representing the rising phase of an ordinary glucose-induced slow oscillation, and the [Ca2+]i, immediately after a transient was lower than just before the spike. These data further emphasize the interplay between voltage-dependent Ca2+-channels and the phospholipase C system in the regulation of B-cell [Ca2+]i-oscillations.

Original languageEnglish
Pages (from-to)85-90
Number of pages6
JournalFEBS Letters
Volume303
Issue number1
DOIs
StatePublished - May 25 1992
Externally publishedYes

Fingerprint

Insulin-Secreting Cells
Protein Kinase C
Cells
Glucose
Type C Phospholipases
B-Lymphocytes
Electric potential

Keywords

  • Pancreatic B-cell
  • Phospholipase C-system
  • Stimulus-secretion coupling, Ca-oscillation
  • Voltage-dependent Ca-channel

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Kindmark, H., Köhler, M., Efendić, S., Rorsman, P., Larsson, O., & Berggren, P. O. (1992). Protein kinase C activity affects glucose-induced oscillations in cytoplasmic free Ca2+ in the pancreatic B-cell. FEBS Letters, 303(1), 85-90. https://doi.org/10.1016/0014-5793(92)80483-W

Protein kinase C activity affects glucose-induced oscillations in cytoplasmic free Ca2+ in the pancreatic B-cell. / Kindmark, Henrik; Köhler, Martin; Efendić, Suad; Rorsman, Patrik; Larsson, Olof; Berggren, Per Olof.

In: FEBS Letters, Vol. 303, No. 1, 25.05.1992, p. 85-90.

Research output: Contribution to journalArticle

Kindmark, H, Köhler, M, Efendić, S, Rorsman, P, Larsson, O & Berggren, PO 1992, 'Protein kinase C activity affects glucose-induced oscillations in cytoplasmic free Ca2+ in the pancreatic B-cell', FEBS Letters, vol. 303, no. 1, pp. 85-90. https://doi.org/10.1016/0014-5793(92)80483-W
Kindmark, Henrik ; Köhler, Martin ; Efendić, Suad ; Rorsman, Patrik ; Larsson, Olof ; Berggren, Per Olof. / Protein kinase C activity affects glucose-induced oscillations in cytoplasmic free Ca2+ in the pancreatic B-cell. In: FEBS Letters. 1992 ; Vol. 303, No. 1. pp. 85-90.
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