TY - JOUR
T1 - Protein kinase C activity affects glucose-induced oscillations in cytoplasmic free Ca2+ in the pancreatic B-cell
AU - Kindmark, Henrik
AU - Köhler, Martin
AU - Efendić, Suad
AU - Rorsman, Patrik
AU - Larsson, Olof
AU - Berggren, Per Olof
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1992/5/25
Y1 - 1992/5/25
N2 - Acute stimulation of protein kinase C (PKC) inhibited glucose-induced slow oscillations in cytoplasmic free Ca2+-concentration, [Ca2+]i, in mouse pancreatic B-cells. In PKC-depleted cells glucose induced rapid transients in [Ca2+]i, lasting for approximately 10 s, superimposed on the slow oscillations in [Ca2+]i. It was demonstrated that the transients did not occur in the absence of extracellular Ca2+. Each transient typically was preceded by a slow increase in [Ca2+]i, representing the rising phase of an ordinary glucose-induced slow oscillation, and the [Ca2+]i, immediately after a transient was lower than just before the spike. These data further emphasize the interplay between voltage-dependent Ca2+-channels and the phospholipase C system in the regulation of B-cell [Ca2+]i-oscillations.
AB - Acute stimulation of protein kinase C (PKC) inhibited glucose-induced slow oscillations in cytoplasmic free Ca2+-concentration, [Ca2+]i, in mouse pancreatic B-cells. In PKC-depleted cells glucose induced rapid transients in [Ca2+]i, lasting for approximately 10 s, superimposed on the slow oscillations in [Ca2+]i. It was demonstrated that the transients did not occur in the absence of extracellular Ca2+. Each transient typically was preceded by a slow increase in [Ca2+]i, representing the rising phase of an ordinary glucose-induced slow oscillation, and the [Ca2+]i, immediately after a transient was lower than just before the spike. These data further emphasize the interplay between voltage-dependent Ca2+-channels and the phospholipase C system in the regulation of B-cell [Ca2+]i-oscillations.
KW - Pancreatic B-cell
KW - Phospholipase C-system
KW - Stimulus-secretion coupling, Ca-oscillation
KW - Voltage-dependent Ca-channel
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U2 - 10.1016/0014-5793(92)80483-W
DO - 10.1016/0014-5793(92)80483-W
M3 - Article
C2 - 1592121
AN - SCOPUS:0026534360
VL - 303
SP - 85
EP - 90
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -