Protein kinase C activity affects glucose-induced oscillations in cytoplasmic free Ca2+ in the pancreatic B-cell

Henrik Kindmark, Martin Köhler, Suad Efendić, Patrik Rorsman, Olof Larsson, Per Olof Berggren

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Acute stimulation of protein kinase C (PKC) inhibited glucose-induced slow oscillations in cytoplasmic free Ca2+-concentration, [Ca2+]i, in mouse pancreatic B-cells. In PKC-depleted cells glucose induced rapid transients in [Ca2+]i, lasting for approximately 10 s, superimposed on the slow oscillations in [Ca2+]i. It was demonstrated that the transients did not occur in the absence of extracellular Ca2+. Each transient typically was preceded by a slow increase in [Ca2+]i, representing the rising phase of an ordinary glucose-induced slow oscillation, and the [Ca2+]i, immediately after a transient was lower than just before the spike. These data further emphasize the interplay between voltage-dependent Ca2+-channels and the phospholipase C system in the regulation of B-cell [Ca2+]i-oscillations.

Original languageEnglish (US)
Pages (from-to)85-90
Number of pages6
JournalFEBS letters
Volume303
Issue number1
DOIs
StatePublished - May 25 1992

Keywords

  • Pancreatic B-cell
  • Phospholipase C-system
  • Stimulus-secretion coupling, Ca-oscillation
  • Voltage-dependent Ca-channel

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Protein kinase C activity affects glucose-induced oscillations in cytoplasmic free Ca<sup>2+</sup> in the pancreatic B-cell'. Together they form a unique fingerprint.

  • Cite this

    Kindmark, H., Köhler, M., Efendić, S., Rorsman, P., Larsson, O., & Berggren, P. O. (1992). Protein kinase C activity affects glucose-induced oscillations in cytoplasmic free Ca2+ in the pancreatic B-cell. FEBS letters, 303(1), 85-90. https://doi.org/10.1016/0014-5793(92)80483-W