Protein immobilization on carbon nanotubes through a molecular adapter

Jeffrey S. Lenihan, Vasilis G. Gavalas, Jianquan Wang, Rodney Andrews, Leonidas G. Bachas

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

A new approach to the modification of carbon nanotubes with biomolecules for the development of nanoscale biosensors is presented. Alkaline phosphatase was immobilized on the surface of multi-wall carbon nanotubes utilizing a layer-by-layer methodology. Carbon nanotubes were incubated with streptavidin, resulting in the formation of a protein layer on the surface of the nanotubes. Biotinylated alkaline phosphatase was then allowed to bind to streptavidin, anchoring the sensing protein onto the surface. Electrochemical biosensors were constructed by using carbon nanotubes compacted into pellets. 1-Naphthyl phosphate, which is hydrolyzed by alkaline phosphatase to the electroactive 1-naphthol, was used as a substrate. Electrodes constructed in this manner were observed to generate an electrochemical signal that was a function of substrate concentration.

Original languageEnglish (US)
Pages (from-to)600-604
Number of pages5
JournalJournal of Nanoscience and Nanotechnology
Volume4
Issue number6
DOIs
StatePublished - Jul 1 2004
Externally publishedYes

Keywords

  • Carbon Nanotubes
  • Layer-by-layer Immobilization
  • Molecular Adapter
  • Nanosensors
  • Protein Functionalization

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

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