Protective effects of amla on oxidative stress and toxicity in rats challenged with dimethyl hydrazine

K. R. Anilakumar, N. S. Nagaraj, K. Santhanam

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The protective effects of amla on oxidative stress and toxicity in rats challenged with dimethyl hydrazine (DMH) were studied. Experiments involving administration of amla at 5% and 10% levels with simultaneous administration of DMH were studied. Amla at both concentrations increased the hepatic GSH and reduced the conjugated dienes. The DMH-induced increase in hepatic malondialdehyde (MDA) was reduced by amla. The co-administration of DMH and 10% amla enhanced the catalase, glutathione peroxidase (GSH-Px) and superoxide dismutase activities in liver. In addition, amla at 10% level per se increased the hepatic ascorbic acid, glutathione (GSH), and antioxidant enzymes while reducing the conjugated dienes, elucidating the protective effect of amla. Amla elevated the colonic glucose-6-phosphate dehydrogenase (G-6-PDH) activity at the 10% level of feeding. The increased GSH associated with GSH-Px may help in reducing the formation of oxidant stress, as observed by the decrease in MDA after amla incorporation. The observed increase in NADPH-dependent glutathione reductase was effective especially in maintaining cellular homeostasis of GSH. It was noted that in rats treated with DMH and fed with 10% amla, the micronuclei formation was reduced by 58% as compared to DMH injected ones. The activity of γ-glutamyl transpeptidase, which was increased significantly (P < 0.001) in kidney upon DMH injection, was reduced by 50% on feeding. amla. The results of this study suggest that amla has the ability to detoxify the DMH partly by enhancing the multicomponent antioxidant system in the rat.

Original languageEnglish (US)
Pages (from-to)313-319
Number of pages7
JournalNutrition Research
Volume24
Issue number4
DOIs
StatePublished - Apr 1 2004
Externally publishedYes

Keywords

  • Amla
  • Antioxidant system
  • Dimethyl hydrazine
  • Lipid peroxides
  • Rat liver

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Nutrition and Dietetics

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