TY - JOUR
T1 - Protective effects of a grape-supplemented diet in a mouse model of retinal degeneration
AU - Patel, Amit K.
AU - Davis, Ashley
AU - Rodriguez, Maria Esperanza
AU - Agron, Samantha
AU - Hackam, Abigail S.
N1 - Funding Information:
This work was supported by the California Table Grape Commission, and institutional support to Bascom Palmer Eye Institute was received from a Research to Prevent Blindness Unrestricted Grant and an NEI Center Core Grant P30 EY014801 . We thank BaoXiang Li and Hany Azcuy for technical support, and William J. Feuer for helpful discussions and statistical analyses. Authors' contributions: conception and design of the study: A.K.P., A.D., and A.S.H. Collection, analysis, and interpretation of data: A.K.P., A.D., M.E.R., S.A., and A.S.H. Manuscript and figures: S.A., A.K.P., and A.S.H. Approval of the final version of the manuscript: A.S.H. Competing interests: the authors declare that they have no competing interests.
PY - 2016
Y1 - 2016
N2 - Objective: Retinal degenerations are a class of devastating blinding diseases that are characterized by photoreceptor dysfunction and death. In this study, we tested whether grape consumption, in the form of freeze-dried grape powder (FDGP), improves photoreceptor survival in a mouse model of retinal degeneration. Methods: Retinal degeneration was induced in mice by acute oxidative stress using subretinal injection of paraquat. The grape-supplemented diet was made by formulating base mouse chow with FDGP, corresponding to three daily human servings of grapes, and a control diet was formulated with equivalent sugar composition as FDGP (0.68% glucose-0.68% fructose mixture). Mice were placed on the diets at weaning for 5 wk before oxidative stress injury until analysis at 2 wk post-injection. Retinal function was measured using electroretinography, thickness of the photoreceptor layer was measured using optical coherence tomography, and rows of photoreceptor nuclei were counted on histologic sections. Results: In mice fed the control diet, oxidative stress significantly reduced photoreceptor layer thickness and photoreceptor numbers. In contrast, retinal thickness and photoreceptor numbers were not reduced by oxidative stress in mice on the grape-supplemented diet, indicating significantly higher photoreceptor survival after injury than mice on the control diet. Furthermore, mice on the grape diet showed preservation of retinal function after oxidative stress injury compared with mice on the control diet. Conclusions: A diet supplemented with grapes rescued retinal structure and function in an oxidative stress-induced mouse model of retinal degeneration, which demonstrates the beneficial effect of grapes on photoreceptors.
AB - Objective: Retinal degenerations are a class of devastating blinding diseases that are characterized by photoreceptor dysfunction and death. In this study, we tested whether grape consumption, in the form of freeze-dried grape powder (FDGP), improves photoreceptor survival in a mouse model of retinal degeneration. Methods: Retinal degeneration was induced in mice by acute oxidative stress using subretinal injection of paraquat. The grape-supplemented diet was made by formulating base mouse chow with FDGP, corresponding to three daily human servings of grapes, and a control diet was formulated with equivalent sugar composition as FDGP (0.68% glucose-0.68% fructose mixture). Mice were placed on the diets at weaning for 5 wk before oxidative stress injury until analysis at 2 wk post-injection. Retinal function was measured using electroretinography, thickness of the photoreceptor layer was measured using optical coherence tomography, and rows of photoreceptor nuclei were counted on histologic sections. Results: In mice fed the control diet, oxidative stress significantly reduced photoreceptor layer thickness and photoreceptor numbers. In contrast, retinal thickness and photoreceptor numbers were not reduced by oxidative stress in mice on the grape-supplemented diet, indicating significantly higher photoreceptor survival after injury than mice on the control diet. Furthermore, mice on the grape diet showed preservation of retinal function after oxidative stress injury compared with mice on the control diet. Conclusions: A diet supplemented with grapes rescued retinal structure and function in an oxidative stress-induced mouse model of retinal degeneration, which demonstrates the beneficial effect of grapes on photoreceptors.
KW - Freeze-dried grape powder
KW - Grapes
KW - Neuroprotection
KW - Nutrition
KW - Oxidative stress
KW - Paraquat
KW - Retina
KW - Retinal degeneration
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U2 - 10.1016/j.nut.2015.09.017
DO - 10.1016/j.nut.2015.09.017
M3 - Article
C2 - 26732835
AN - SCOPUS:84961214841
VL - 32
SP - 384
EP - 390
JO - Nutrition
JF - Nutrition
SN - 0899-9007
IS - 3
ER -