Prostate-Specific Natural Health Products (Dietary Supplements) Radiosensitize Normal Prostate Cells

Yasmin Hasan, Diane Schoenherr, Alvaro A. Martinez, George D. Wilson, Brian Marples

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: Prostate-specific health products (dietary supplements) are taken by cancer patients to alleviate the symptoms linked with poor prostate health. However, the effect of these agents on evidence-based radiotherapy practice is poorly understood. The present study aimed to determine whether dietary supplements radiosensitized normal prostate or prostate cancer cell lines. Methods and Materials: Three well-known prostate-specific dietary supplements were purchased from commercial sources available to patients (Trinovin, Provelex, and Prostate Rx). The cells used in the study included normal prostate lines (RWPE-1 and PWR-1E), prostate tumor lines (PC3, DU145, and LNCaP), and a normal nonprostate line (HaCaT). Supplement toxicity was assessed using cell proliferation assays [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and cellular radiosensitivity using conventional clonogenic assays (0.5-4Gy). Cell cycle kinetics were assessed using the bromodeoxyuridine/propidium iodide pulse-labeling technique, apoptosis by scoring caspase-3 activation, and DNA repair by assessing γH2AX. Results: The cell growth and radiosensitivity of the malignant PC3, DU145, and LNcaP cells were not affected by any of the dietary prostate supplements (Provelex [2μg/mL], Trinovin [10μg/mL], and Prostate Rx [50 μg/mL]). However, both Trinovin (10μg/mL) and Prostate Rx (6μg/mL) inhibited the growth rate of the normal prostate cell lines. Prostate Rx increased cellular radiosensitivity of RWPE-1 cells through the inhibition of DNA repair. Conclusion: The use of prostate-specific dietary supplements should be discouraged during radiotherapy owing to the preferential radiosensitization of normal prostate cells.

Original languageEnglish (US)
Pages (from-to)896-904
Number of pages9
JournalInternational Journal of Radiation Oncology Biology Physics
Volume76
Issue number3
DOIs
StatePublished - Mar 1 2010
Externally publishedYes

Fingerprint

supplements
Dietary Supplements
health
Prostate
Health
radiation tolerance
products
cells
cultured cells
radiation therapy
deoxyribonucleic acid
cancer
Radiation Tolerance
scoring
apoptosis
toxicity
iodides
marking
bromides
DNA Repair

Keywords

  • Dietary supplements
  • Ionizing radiation
  • Prostate
  • Radiosensitivity

ASJC Scopus subject areas

  • Radiation
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Prostate-Specific Natural Health Products (Dietary Supplements) Radiosensitize Normal Prostate Cells. / Hasan, Yasmin; Schoenherr, Diane; Martinez, Alvaro A.; Wilson, George D.; Marples, Brian.

In: International Journal of Radiation Oncology Biology Physics, Vol. 76, No. 3, 01.03.2010, p. 896-904.

Research output: Contribution to journalArticle

Hasan, Yasmin ; Schoenherr, Diane ; Martinez, Alvaro A. ; Wilson, George D. ; Marples, Brian. / Prostate-Specific Natural Health Products (Dietary Supplements) Radiosensitize Normal Prostate Cells. In: International Journal of Radiation Oncology Biology Physics. 2010 ; Vol. 76, No. 3. pp. 896-904.
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