Prostate cancer progression, metastasis, and gene expression in transgenic mice

Carlos Perez-Stable, Norman H. Altman, Parmender P. Mehta, Leonard J. Deftos, Bernard A. Roos

Research output: Contribution to journalArticle

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Abstract

We previously reported that a transgenic mouse line containing the fetal globin promoter linked to the SV40 T antigen (T Ag) viral oncogene (Gγ/T- 15) resulted in prostate tumors. In this study, we further explored tumor origin, frequency, invasiveness, androgen sensitivity, and gene expression pattern. T Ag was detected in adult but not fetal and neonatal prostates, suggesting a role for androgens in tumor progression. However, castration shortly after prostate morphogenesis did not prevent tumor development, suggesting an androgen-independent phenotype. Tumors originated within ventral or dorsal prostate lobes and involved intraepithelial neoplasia, rapid growth in the pelvic region, and metastasis to lymph nodes and distant sites. In addition, the primary cancers could be propagated in nude mice or nontransgenic mice. Seventy-five percent of hemizygous and 100% of homozygous transgenic males developed prostate tumors, suggesting a T Ag dosage effect. Biochemical characterization of advanced tumors revealed markers of both neuroendocrine and epithelial phenotypes; markers of terminal differentiation are lost early in tumorigenesis. Tumor suppressor genes (p53 and Rb), normally bound to T Ag, were up-regulated; bcl-2 proto-oncogene, which prevents apoptosis, was slightly up-regulated. Myc, a stimulus to cell cycle progression, was unchanged. We propose the Gγ/T-15 transgenic line as a model of highly aggressive androgen-independent metastatic prostate carcinoma with features similar to end-stage prostate cancer in humans.

Original languageEnglish
Pages (from-to)900-906
Number of pages7
JournalCancer Research
Volume57
Issue number5
StatePublished - Mar 11 1997

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Transgenic Mice
Prostatic Neoplasms
Neoplasm Metastasis
Gene Expression
Prostate
Androgens
Viral Tumor Antigens
Neoplasms
Polyomavirus Transforming Antigens
Phenotype
Globins
Proto-Oncogenes
Castration
Differentiation Antigens
Tumor Biomarkers
Tumor Suppressor Genes
Pelvis
Morphogenesis
Oncogenes
Nude Mice

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Perez-Stable, C., Altman, N. H., Mehta, P. P., Deftos, L. J., & Roos, B. A. (1997). Prostate cancer progression, metastasis, and gene expression in transgenic mice. Cancer Research, 57(5), 900-906.

Prostate cancer progression, metastasis, and gene expression in transgenic mice. / Perez-Stable, Carlos; Altman, Norman H.; Mehta, Parmender P.; Deftos, Leonard J.; Roos, Bernard A.

In: Cancer Research, Vol. 57, No. 5, 11.03.1997, p. 900-906.

Research output: Contribution to journalArticle

Perez-Stable, C, Altman, NH, Mehta, PP, Deftos, LJ & Roos, BA 1997, 'Prostate cancer progression, metastasis, and gene expression in transgenic mice', Cancer Research, vol. 57, no. 5, pp. 900-906.
Perez-Stable C, Altman NH, Mehta PP, Deftos LJ, Roos BA. Prostate cancer progression, metastasis, and gene expression in transgenic mice. Cancer Research. 1997 Mar 11;57(5):900-906.
Perez-Stable, Carlos ; Altman, Norman H. ; Mehta, Parmender P. ; Deftos, Leonard J. ; Roos, Bernard A. / Prostate cancer progression, metastasis, and gene expression in transgenic mice. In: Cancer Research. 1997 ; Vol. 57, No. 5. pp. 900-906.
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