Prostaglandin (PG) E2 generation by cultured canine synovial fibroblasts exposed to microcrystals containing calcium

D. J. McCarty, Herman S Cheung

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Immunoreactive prostaglandin (PG) E2 was released into the ambient medium in a dose dependent fashion when either hydroxyapatite (HA) or calcium pyrophosphate dihydrate (CPPD) crystals were added to canine synovial fibroblasts in tissue culture. PGE2 release peaked 6 to 9 hours after HA or CPPD crystals were added in the presence of serum but at 24 hours if they were added in the presence of lactalbumin hydrolysate. PGE2 release correlated with crystal endocytosis estimated by serial phase contrast microscopy and time lapse photography. As postulated previously by others for monosodium urate crystals, prostaglandin production by synovial cells may also be related to the pathogenesis of the destructive arthropathies associated with HA or CPPD crystals.

Original languageEnglish
Pages (from-to)316-320
Number of pages5
JournalAnnals of the Rheumatic Diseases
Volume44
Issue number5
StatePublished - Jan 1 1985
Externally publishedYes

Fingerprint

Calcium Pyrophosphate
Microcrystals
Durapatite
Fibroblasts
Dinoprostone
Canidae
Calcium
Crystals
Phase-Contrast Microscopy
Lactalbumin
Joint Diseases
Photography
Endocytosis
Uric Acid
Prostaglandins
Special effects
Tissue culture
Serum
Microscopic examination

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Prostaglandin (PG) E2 generation by cultured canine synovial fibroblasts exposed to microcrystals containing calcium. / McCarty, D. J.; Cheung, Herman S.

In: Annals of the Rheumatic Diseases, Vol. 44, No. 5, 01.01.1985, p. 316-320.

Research output: Contribution to journalArticle

@article{cb28d902a8864552b8a313a837c44941,
title = "Prostaglandin (PG) E2 generation by cultured canine synovial fibroblasts exposed to microcrystals containing calcium",
abstract = "Immunoreactive prostaglandin (PG) E2 was released into the ambient medium in a dose dependent fashion when either hydroxyapatite (HA) or calcium pyrophosphate dihydrate (CPPD) crystals were added to canine synovial fibroblasts in tissue culture. PGE2 release peaked 6 to 9 hours after HA or CPPD crystals were added in the presence of serum but at 24 hours if they were added in the presence of lactalbumin hydrolysate. PGE2 release correlated with crystal endocytosis estimated by serial phase contrast microscopy and time lapse photography. As postulated previously by others for monosodium urate crystals, prostaglandin production by synovial cells may also be related to the pathogenesis of the destructive arthropathies associated with HA or CPPD crystals.",
author = "McCarty, {D. J.} and Cheung, {Herman S}",
year = "1985",
month = "1",
day = "1",
language = "English",
volume = "44",
pages = "316--320",
journal = "Annals of the Rheumatic Diseases",
issn = "0003-4967",
publisher = "BMJ Publishing Group",
number = "5",

}

TY - JOUR

T1 - Prostaglandin (PG) E2 generation by cultured canine synovial fibroblasts exposed to microcrystals containing calcium

AU - McCarty, D. J.

AU - Cheung, Herman S

PY - 1985/1/1

Y1 - 1985/1/1

N2 - Immunoreactive prostaglandin (PG) E2 was released into the ambient medium in a dose dependent fashion when either hydroxyapatite (HA) or calcium pyrophosphate dihydrate (CPPD) crystals were added to canine synovial fibroblasts in tissue culture. PGE2 release peaked 6 to 9 hours after HA or CPPD crystals were added in the presence of serum but at 24 hours if they were added in the presence of lactalbumin hydrolysate. PGE2 release correlated with crystal endocytosis estimated by serial phase contrast microscopy and time lapse photography. As postulated previously by others for monosodium urate crystals, prostaglandin production by synovial cells may also be related to the pathogenesis of the destructive arthropathies associated with HA or CPPD crystals.

AB - Immunoreactive prostaglandin (PG) E2 was released into the ambient medium in a dose dependent fashion when either hydroxyapatite (HA) or calcium pyrophosphate dihydrate (CPPD) crystals were added to canine synovial fibroblasts in tissue culture. PGE2 release peaked 6 to 9 hours after HA or CPPD crystals were added in the presence of serum but at 24 hours if they were added in the presence of lactalbumin hydrolysate. PGE2 release correlated with crystal endocytosis estimated by serial phase contrast microscopy and time lapse photography. As postulated previously by others for monosodium urate crystals, prostaglandin production by synovial cells may also be related to the pathogenesis of the destructive arthropathies associated with HA or CPPD crystals.

UR - http://www.scopus.com/inward/record.url?scp=0021894652&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021894652&partnerID=8YFLogxK

M3 - Article

C2 - 2988464

AN - SCOPUS:0021894652

VL - 44

SP - 316

EP - 320

JO - Annals of the Rheumatic Diseases

JF - Annals of the Rheumatic Diseases

SN - 0003-4967

IS - 5

ER -