Prospective cohort study of microbial and inflammatory events immediately preceding escherichia coli recurrent urinary tract infection in women

C. A. Czaja, W. E. Stamm, A. E. Stapleton, P. L. Roberts, T. R. Hawn, D. Scholes, M. Samadpour, S. J. Hultgren, Thomas Hooton

Research output: Contribution to journalArticle

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Abstract

Background. A prospective cohort study was conducted to characterize the temporal sequence of microbial and inflammatory events immediately preceding Escherichia coli recurrent urinary tract infection (rUTI). Methods. Women with acute cystitis and a history of UTI within the previous year self-collected periurethral and urine samples daily and recorded measurements of urine leukocyte esterase, symptoms, and sexual intercourse daily for 3 months. rUTI strains were characterized by pulsed-field gel electrophoresis and genomic virulence profiling. Urinary cytokine levels were measured. Results. There were 38 E. coli rUTIs in 29 of 104 women. The prevalence of periurethral rUTI strain carriage increased from 46% to 90% during the 14 days immediately preceding rUTI, with similar increases in same-strain bacteriuria (from 7% to 69%), leukocyte esterase (from 31% to 64%), and symptoms (from 3% to 43%), most notably 2-3 days before rUTI (P<.05 for all comparisons). Intercourse with periurethral carriage of the rUTI strain also increased before rUTI (P = .008). Recurrent UTIs preceded by bacteriuria, pyuria, and symptoms were caused by strains less likely to have P fimbriae than other rUTI strains (P = .002). Conclusions. Among women with frequent rUTIs, the prevalences of periurethral rUTI strain carriage, bacteriuria, pyuria, and intercourse dramatically increase over the days preceding rUTI. A better understanding of the pathogenesis of rUTI will lead to better prevention strategies.

Original languageEnglish
Pages (from-to)528-536
Number of pages9
JournalJournal of Infectious Diseases
Volume200
Issue number4
DOIs
StatePublished - Aug 15 2009

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Urinary Tract Infections
Cohort Studies
Prospective Studies
Escherichia coli
Bacteriuria
Pyuria
Urine
Cystitis
Pulsed Field Gel Electrophoresis
Coitus
Virulence
Cytokines

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

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Prospective cohort study of microbial and inflammatory events immediately preceding escherichia coli recurrent urinary tract infection in women. / Czaja, C. A.; Stamm, W. E.; Stapleton, A. E.; Roberts, P. L.; Hawn, T. R.; Scholes, D.; Samadpour, M.; Hultgren, S. J.; Hooton, Thomas.

In: Journal of Infectious Diseases, Vol. 200, No. 4, 15.08.2009, p. 528-536.

Research output: Contribution to journalArticle

Czaja, C. A. ; Stamm, W. E. ; Stapleton, A. E. ; Roberts, P. L. ; Hawn, T. R. ; Scholes, D. ; Samadpour, M. ; Hultgren, S. J. ; Hooton, Thomas. / Prospective cohort study of microbial and inflammatory events immediately preceding escherichia coli recurrent urinary tract infection in women. In: Journal of Infectious Diseases. 2009 ; Vol. 200, No. 4. pp. 528-536.
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abstract = "Background. A prospective cohort study was conducted to characterize the temporal sequence of microbial and inflammatory events immediately preceding Escherichia coli recurrent urinary tract infection (rUTI). Methods. Women with acute cystitis and a history of UTI within the previous year self-collected periurethral and urine samples daily and recorded measurements of urine leukocyte esterase, symptoms, and sexual intercourse daily for 3 months. rUTI strains were characterized by pulsed-field gel electrophoresis and genomic virulence profiling. Urinary cytokine levels were measured. Results. There were 38 E. coli rUTIs in 29 of 104 women. The prevalence of periurethral rUTI strain carriage increased from 46{\%} to 90{\%} during the 14 days immediately preceding rUTI, with similar increases in same-strain bacteriuria (from 7{\%} to 69{\%}), leukocyte esterase (from 31{\%} to 64{\%}), and symptoms (from 3{\%} to 43{\%}), most notably 2-3 days before rUTI (P<.05 for all comparisons). Intercourse with periurethral carriage of the rUTI strain also increased before rUTI (P = .008). Recurrent UTIs preceded by bacteriuria, pyuria, and symptoms were caused by strains less likely to have P fimbriae than other rUTI strains (P = .002). Conclusions. Among women with frequent rUTIs, the prevalences of periurethral rUTI strain carriage, bacteriuria, pyuria, and intercourse dramatically increase over the days preceding rUTI. A better understanding of the pathogenesis of rUTI will lead to better prevention strategies.",
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AU - Stamm, W. E.

AU - Stapleton, A. E.

AU - Roberts, P. L.

AU - Hawn, T. R.

AU - Scholes, D.

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AU - Hultgren, S. J.

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N2 - Background. A prospective cohort study was conducted to characterize the temporal sequence of microbial and inflammatory events immediately preceding Escherichia coli recurrent urinary tract infection (rUTI). Methods. Women with acute cystitis and a history of UTI within the previous year self-collected periurethral and urine samples daily and recorded measurements of urine leukocyte esterase, symptoms, and sexual intercourse daily for 3 months. rUTI strains were characterized by pulsed-field gel electrophoresis and genomic virulence profiling. Urinary cytokine levels were measured. Results. There were 38 E. coli rUTIs in 29 of 104 women. The prevalence of periurethral rUTI strain carriage increased from 46% to 90% during the 14 days immediately preceding rUTI, with similar increases in same-strain bacteriuria (from 7% to 69%), leukocyte esterase (from 31% to 64%), and symptoms (from 3% to 43%), most notably 2-3 days before rUTI (P<.05 for all comparisons). Intercourse with periurethral carriage of the rUTI strain also increased before rUTI (P = .008). Recurrent UTIs preceded by bacteriuria, pyuria, and symptoms were caused by strains less likely to have P fimbriae than other rUTI strains (P = .002). Conclusions. Among women with frequent rUTIs, the prevalences of periurethral rUTI strain carriage, bacteriuria, pyuria, and intercourse dramatically increase over the days preceding rUTI. A better understanding of the pathogenesis of rUTI will lead to better prevention strategies.

AB - Background. A prospective cohort study was conducted to characterize the temporal sequence of microbial and inflammatory events immediately preceding Escherichia coli recurrent urinary tract infection (rUTI). Methods. Women with acute cystitis and a history of UTI within the previous year self-collected periurethral and urine samples daily and recorded measurements of urine leukocyte esterase, symptoms, and sexual intercourse daily for 3 months. rUTI strains were characterized by pulsed-field gel electrophoresis and genomic virulence profiling. Urinary cytokine levels were measured. Results. There were 38 E. coli rUTIs in 29 of 104 women. The prevalence of periurethral rUTI strain carriage increased from 46% to 90% during the 14 days immediately preceding rUTI, with similar increases in same-strain bacteriuria (from 7% to 69%), leukocyte esterase (from 31% to 64%), and symptoms (from 3% to 43%), most notably 2-3 days before rUTI (P<.05 for all comparisons). Intercourse with periurethral carriage of the rUTI strain also increased before rUTI (P = .008). Recurrent UTIs preceded by bacteriuria, pyuria, and symptoms were caused by strains less likely to have P fimbriae than other rUTI strains (P = .002). Conclusions. Among women with frequent rUTIs, the prevalences of periurethral rUTI strain carriage, bacteriuria, pyuria, and intercourse dramatically increase over the days preceding rUTI. A better understanding of the pathogenesis of rUTI will lead to better prevention strategies.

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