Prophylactic and antinociceptive effects of coenzyme q10 on diabetic neuropathic pain in a mouse model of type 1 diabetes

Yan Ping Zhang, Ariel Eber, Yue Yuan, Zhe Yang, Yiliam Rodriguez, Roy C Levitt, Peter Takacs, Keith A Candiotti

Research output: Contribution to journalArticle

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Abstract

Background: Oxidative stress is a key factor implicated in the development of diabetic neuropathy. This study evaluates the prophylactic and antinociceptive effects of the antioxidant coenzyme Q10 (CoQ10) on diabetes-induced neuropathic pain in a diabetic mouse model. Methods: Total 56 mice with type 1 diabetes induced by streptozotocin were used, 20 normal mice were used as control. Mechanical and thermal nociceptive behavioral assays were applied to evaluate diabetic neuropathic pain. Tissue lipid peroxidation, immunohistochemistry, reverse transcription, and polymerase chain reaction were used to evaluate the molecular mechanisms of CoQ10. Data are presented as mean ± SEM. Results: CoQ10 administration was associated with reduced loss of body weight compared with nontreated diabetic mice, without affecting blood glucose levels. Low dose and long-term administration of CoQ10 prevented the development of neuropathic pain. Treatment with CoQ10 produced a significant dose-dependent inhibition of mechanical allodynia and thermal hyperalgesia in diabetic mice. Dorsal root ganglia, sciatic nerve, and spinal cord tissues from diabetic mice demonstrated increased lipid peroxidation that was reduced by CoQ10 treatment. CoQ10 administration was also noted to reduce the proinflammatory factors in the peripheral and central nervous system. Conclusions: The results of this study support the hypothesis that hyperglycemia induced neuronal oxidative damage and reactive inflammation may be pathogenic in diabetic neuropathic pain. CoQ10 may be protective by inhibiting oxidative stress and reducing inflammation by down-regulating proinflammatory factors. These results suggest that CoQ10 administration may represent a low-risk, high-reward strategy for preventing or treating diabetic neuropathy.

Original languageEnglish
Pages (from-to)945-954
Number of pages10
JournalAnesthesiology
Volume118
Issue number4
DOIs
StatePublished - Apr 1 2013

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coenzyme Q10
Neuralgia
Type 1 Diabetes Mellitus
Hyperalgesia
Diabetic Neuropathies
Lipid Peroxidation
Oxidative Stress
Inflammation
Peripheral Nervous System
Spinal Ganglia
Sciatic Nerve
Streptozocin
Reward

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Prophylactic and antinociceptive effects of coenzyme q10 on diabetic neuropathic pain in a mouse model of type 1 diabetes. / Zhang, Yan Ping; Eber, Ariel; Yuan, Yue; Yang, Zhe; Rodriguez, Yiliam; Levitt, Roy C; Takacs, Peter; Candiotti, Keith A.

In: Anesthesiology, Vol. 118, No. 4, 01.04.2013, p. 945-954.

Research output: Contribution to journalArticle

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abstract = "Background: Oxidative stress is a key factor implicated in the development of diabetic neuropathy. This study evaluates the prophylactic and antinociceptive effects of the antioxidant coenzyme Q10 (CoQ10) on diabetes-induced neuropathic pain in a diabetic mouse model. Methods: Total 56 mice with type 1 diabetes induced by streptozotocin were used, 20 normal mice were used as control. Mechanical and thermal nociceptive behavioral assays were applied to evaluate diabetic neuropathic pain. Tissue lipid peroxidation, immunohistochemistry, reverse transcription, and polymerase chain reaction were used to evaluate the molecular mechanisms of CoQ10. Data are presented as mean ± SEM. Results: CoQ10 administration was associated with reduced loss of body weight compared with nontreated diabetic mice, without affecting blood glucose levels. Low dose and long-term administration of CoQ10 prevented the development of neuropathic pain. Treatment with CoQ10 produced a significant dose-dependent inhibition of mechanical allodynia and thermal hyperalgesia in diabetic mice. Dorsal root ganglia, sciatic nerve, and spinal cord tissues from diabetic mice demonstrated increased lipid peroxidation that was reduced by CoQ10 treatment. CoQ10 administration was also noted to reduce the proinflammatory factors in the peripheral and central nervous system. Conclusions: The results of this study support the hypothesis that hyperglycemia induced neuronal oxidative damage and reactive inflammation may be pathogenic in diabetic neuropathic pain. CoQ10 may be protective by inhibiting oxidative stress and reducing inflammation by down-regulating proinflammatory factors. These results suggest that CoQ10 administration may represent a low-risk, high-reward strategy for preventing or treating diabetic neuropathy.",
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