Propagation, expansion, and multilineage differentiation of human somatic stem cells from dermal progenitors

Georg Bartsch, James J. Yoo, Paolo De Coppi, M. Minhaj Siddiqui, Gunter Schuch, Hans G. Pohl, Jasmin Fuhr, Laura Perin, Shay Soker, Anthony Atala

Research output: Contribution to journalArticle

113 Scopus citations

Abstract

We isolated multipotential mesenchymal stem cells (MSC) from human postnatal dermal tissues. The isolated cells were expanded and maintained for over 100 population doublings with retention of their chromosomal complement and potential for multilineage differentiation. Progeny of cell lines established from a single dermal MSC could be differentiated into adipogenic, osteogenic, and myogenic lineages, consistent with the conclusion that we established a clonal, multipotential, somatic MSC cell line. This study is the first to show potential growth of multipotential human MSC cell lines from a single cell that can be used for the engineering of tissues in vivo. Clonal growth of MSCs presents profound implications in our understanding of differentiation and development, and should provide a valuable resource for tissue repair.

Original languageEnglish (US)
Pages (from-to)337-348
Number of pages12
JournalStem cells and development
Volume14
Issue number3
DOIs
StatePublished - Jun 1 2005

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

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    Bartsch, G., Yoo, J. J., De Coppi, P., Siddiqui, M. M., Schuch, G., Pohl, H. G., Fuhr, J., Perin, L., Soker, S., & Atala, A. (2005). Propagation, expansion, and multilineage differentiation of human somatic stem cells from dermal progenitors. Stem cells and development, 14(3), 337-348. https://doi.org/10.1089/scd.2005.14.337