Propagation, expansion, and multilineage differentiation of human somatic stem cells from dermal progenitors

Georg Bartsch, James J. Yoo, Paolo De Coppi, M. Minhaj Siddiqui, Gunter Schuch, Hans G. Pohl, Jasmin Fuhr, Laura Perin, Shay Soker, Anthony Atala

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

We isolated multipotential mesenchymal stem cells (MSC) from human postnatal dermal tissues. The isolated cells were expanded and maintained for over 100 population doublings with retention of their chromosomal complement and potential for multilineage differentiation. Progeny of cell lines established from a single dermal MSC could be differentiated into adipogenic, osteogenic, and myogenic lineages, consistent with the conclusion that we established a clonal, multipotential, somatic MSC cell line. This study is the first to show potential growth of multipotential human MSC cell lines from a single cell that can be used for the engineering of tissues in vivo. Clonal growth of MSCs presents profound implications in our understanding of differentiation and development, and should provide a valuable resource for tissue repair.

Original languageEnglish
Pages (from-to)337-348
Number of pages12
JournalStem Cells and Development
Volume14
Issue number3
DOIs
StatePublished - Jun 1 2005
Externally publishedYes

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Adult Stem Cells
Mesenchymal Stromal Cells
Skin
Cell Line
Tissue Engineering
Growth
Population

ASJC Scopus subject areas

  • Hematology

Cite this

Bartsch, G., Yoo, J. J., De Coppi, P., Siddiqui, M. M., Schuch, G., Pohl, H. G., ... Atala, A. (2005). Propagation, expansion, and multilineage differentiation of human somatic stem cells from dermal progenitors. Stem Cells and Development, 14(3), 337-348. https://doi.org/10.1089/scd.2005.14.337

Propagation, expansion, and multilineage differentiation of human somatic stem cells from dermal progenitors. / Bartsch, Georg; Yoo, James J.; De Coppi, Paolo; Siddiqui, M. Minhaj; Schuch, Gunter; Pohl, Hans G.; Fuhr, Jasmin; Perin, Laura; Soker, Shay; Atala, Anthony.

In: Stem Cells and Development, Vol. 14, No. 3, 01.06.2005, p. 337-348.

Research output: Contribution to journalArticle

Bartsch, G, Yoo, JJ, De Coppi, P, Siddiqui, MM, Schuch, G, Pohl, HG, Fuhr, J, Perin, L, Soker, S & Atala, A 2005, 'Propagation, expansion, and multilineage differentiation of human somatic stem cells from dermal progenitors', Stem Cells and Development, vol. 14, no. 3, pp. 337-348. https://doi.org/10.1089/scd.2005.14.337
Bartsch, Georg ; Yoo, James J. ; De Coppi, Paolo ; Siddiqui, M. Minhaj ; Schuch, Gunter ; Pohl, Hans G. ; Fuhr, Jasmin ; Perin, Laura ; Soker, Shay ; Atala, Anthony. / Propagation, expansion, and multilineage differentiation of human somatic stem cells from dermal progenitors. In: Stem Cells and Development. 2005 ; Vol. 14, No. 3. pp. 337-348.
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