Promoter hypermethylation and suppression of glutathione peroxidase 3 are associated with inflammatory breast carcinogenesis

Mona M. Mohamed, Salwa Sabet, Dunfa Peng, M. Akram Nouh, Mohamed El-Shinawi, Wael El-Rifai

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) play a crucial role in breast cancer initiation, promotion, and progression. Inhibition of antioxidant enzymes that remove ROS was found to accelerate cancer growth. Studies showed that inhibition of glutathione peroxidase-3 (GPX3) was associated with cancer progression. Although the role of GPX3 has been studied in different cancer types, its role in breast cancer and its epigenetic regulation have not yet been investigated. The aim of the present study was to investigate GPX3 expression and epigenetic regulation in carcinoma tissues of breast cancer patients' in comparison to normal breast tissues. Furthermore, we compared GPX3 level of expression and methylation status in aggressive phenotype inflammatory breast cancer (IBC) versus non-IBC invasive ductal carcinoma (IDC). We found that GPX3 mRNA and protein expression levels were downregulated in the carcinoma tissues of IBC compared to non-IBC. However, we did not detect significant correlation between GPX3 and patients' clinical-pathological prosperities. Promoter hypermethylation of GPX3 gene was detected in carcinoma tissues not normal breast tissues. In addition, IBC carcinoma tissues showed a significant increase in the promoter hypermethylation of GPX3 gene compared to non-IBC. Our results propose that downregulation of GPX3 in IBC may play a role in the disease progression.

Original languageEnglish (US)
Article number787195
JournalOxidative Medicine and Cellular Longevity
Volume2014
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

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Glutathione Peroxidase
Carcinogenesis
Inflammatory Breast Neoplasms
Breast
Tissue
Breast Neoplasms
Carcinoma
Epigenomics
Reactive Oxygen Species
Down-Regulation
Genes
Carcinoma, Ductal, Breast
Neoplasms
Methylation
Disease Progression
Antioxidants
Phenotype
Messenger RNA
Enzymes
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Cell Biology

Cite this

Promoter hypermethylation and suppression of glutathione peroxidase 3 are associated with inflammatory breast carcinogenesis. / Mohamed, Mona M.; Sabet, Salwa; Peng, Dunfa; Nouh, M. Akram; El-Shinawi, Mohamed; El-Rifai, Wael.

In: Oxidative Medicine and Cellular Longevity, Vol. 2014, 787195, 01.01.2014.

Research output: Contribution to journalArticle

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