Prominent sex steroid metabolism in human lymphocytes

Zhifeng Zhou, Cedric H L Shackleton, Savita G Pahwa, Perrin C. White, Phyllis W. Speiser

Research output: Contribution to journalArticle

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Abstract

Steroid metabolism was investigated in cultured human B-lymphoblastoid cells (B-LCL), and peripheral blood T and B cells. Gene expression was examined by reverse-transcription polymerase chain reaction amplification (RT-PCR). Appropriate sized transcripts were detected in both cultured and fresh peripheral lymphocytes for CYP11A, CYP17, HSD11L (11β-hydroxysteroid dehydrogenase I), HSD17B1 (17β-hydroxysteroid dehydrogenase type I) and SRD5A1 (5α-reductase I). B-LCL, but not T and B cells, expressed CYP11B. There was minimal expression of HSD3B1 and HSD3B2 (3β-hydroxysteroid dehydrogenase I and II) in B-LCL and T cells. Transcripts for CYP19 and HSD11K were not detected. Corresponding enzymatic activity was detectable only for 17-hydroxysteroid dehydrogenase and 5α-reductase, respectively producing testosterone and 5α-dihydrotestosterone. Steroid identities were confirmed by gas chromatography/mass spectrometry (GC/MS). One metabolite thought to be deoxycorticosterone was identified by GC/MS as 6α-hydroxypregnanolone. It was concluded that sex hormone metabolism, including androgen synthesis, occurs in lymphocytes, and may modulate immune response.

Original languageEnglish
Pages (from-to)61-69
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume138
Issue number1-2
DOIs
StatePublished - Mar 16 1998
Externally publishedYes

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Lymphocytes
Metabolism
Gas chromatography
Mass spectrometry
Oxidoreductases
3-Hydroxysteroid Dehydrogenases
Steroids
Cells
11-beta-Hydroxysteroid Dehydrogenases
Steroid 17-alpha-Hydroxylase
T-Lymphocytes
Desoxycorticosterone
Gas Chromatography-Mass Spectrometry
Aromatase
T-cells
Dihydrotestosterone
Polymerase chain reaction
Gonadal Steroid Hormones
B-Lymphocytes
Transcription

Keywords

  • Androgens
  • Lymphocytes
  • Steroid 17 hydroxysteroid dehydrogenase
  • Steroid 5 alpha reductase

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Prominent sex steroid metabolism in human lymphocytes. / Zhou, Zhifeng; Shackleton, Cedric H L; Pahwa, Savita G; White, Perrin C.; Speiser, Phyllis W.

In: Molecular and Cellular Endocrinology, Vol. 138, No. 1-2, 16.03.1998, p. 61-69.

Research output: Contribution to journalArticle

Zhou, Zhifeng ; Shackleton, Cedric H L ; Pahwa, Savita G ; White, Perrin C. ; Speiser, Phyllis W. / Prominent sex steroid metabolism in human lymphocytes. In: Molecular and Cellular Endocrinology. 1998 ; Vol. 138, No. 1-2. pp. 61-69.
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