Promatrilysin expression is induced by fibroblast growth factors in the prostatic carcinoma cell line LNCaP but not in normal primary prostate epithelial cells

Russell D. Klein, M. Suzanne Maliner-Jongewaard, Thirupandiyur Udayakumar, Jeff L. Boyd, Raymond B. Nagle, G. Tim Bowden

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

BACKGROUND. It has been determined that prostate cancer cells overexpress the matrix metalloprotease matrilysin (MMP-7), but the factors regulating this expression have not been identified. Fibroblast growth factors (FGF); which are expressed in the prostate, might participate in paracrine regulation of matrilysin expression by prostate cancer cells. METHODS. We tested the ability of recombinant FGF proteins and prostate fibroblast-conditioned media (PFCM) to induce promatrilysin expression in the prostate carcinoma cell line, LNCaP, and in normal prostate epithelial (PrEC) cells. We also characterized prostate fibroblast FGF expression by reverse transcriptase-polymerase chain reaction (RT-PCR). An inhibitor of FGF receptor activation (SU5402) was used to determine the role of FGF proteins in the induction of promatrilysin expression by PFCM. RESULTS. Recombinant FGF-1, FGF-2, FGF-9, FGF-10, and PFCM significantly induced promatrilysin expression in LNCaP cells but not in PrEC cells. Prostate fibroblasts express mRNAs for these FGF proteins, and inhibition of LNCaP cell FGF receptors with SU5402 substantially reduced the induction of promatrilysin expression by PFCM. CONCLUSIONS. Stromally expressed FGF proteins induce promatrilysin expression in a prostate carcinoma cell, and may provide a mechanism for the overexpression of promatrilysin observed in prostate cancer.

Original languageEnglish (US)
Pages (from-to)215-223
Number of pages9
JournalProstate
Volume41
Issue number4
DOIs
StatePublished - 1999
Externally publishedYes

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Fibroblast Growth Factors
Prostate
Epithelial Cells
Carcinoma
Cell Line
Fibroblasts
Conditioned Culture Medium
Matrix Metalloproteinase 7
Fibroblast Growth Factor Receptors
Prostatic Neoplasms
Fibroblast Growth Factor 9
Proteins
Fibroblast Growth Factor 10
promatrilysin
Fibroblast Growth Factor 1
Metalloproteases
Fibroblast Growth Factor 2
Reverse Transcriptase Polymerase Chain Reaction
Matrix Metalloproteinases
Messenger RNA

Keywords

  • FGF
  • Invasion
  • Metalloprotease

ASJC Scopus subject areas

  • Urology

Cite this

Promatrilysin expression is induced by fibroblast growth factors in the prostatic carcinoma cell line LNCaP but not in normal primary prostate epithelial cells. / Klein, Russell D.; Maliner-Jongewaard, M. Suzanne; Udayakumar, Thirupandiyur; Boyd, Jeff L.; Nagle, Raymond B.; Bowden, G. Tim.

In: Prostate, Vol. 41, No. 4, 1999, p. 215-223.

Research output: Contribution to journalArticle

Klein, Russell D. ; Maliner-Jongewaard, M. Suzanne ; Udayakumar, Thirupandiyur ; Boyd, Jeff L. ; Nagle, Raymond B. ; Bowden, G. Tim. / Promatrilysin expression is induced by fibroblast growth factors in the prostatic carcinoma cell line LNCaP but not in normal primary prostate epithelial cells. In: Prostate. 1999 ; Vol. 41, No. 4. pp. 215-223.
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T1 - Promatrilysin expression is induced by fibroblast growth factors in the prostatic carcinoma cell line LNCaP but not in normal primary prostate epithelial cells

AU - Klein, Russell D.

AU - Maliner-Jongewaard, M. Suzanne

AU - Udayakumar, Thirupandiyur

AU - Boyd, Jeff L.

AU - Nagle, Raymond B.

AU - Bowden, G. Tim

PY - 1999

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N2 - BACKGROUND. It has been determined that prostate cancer cells overexpress the matrix metalloprotease matrilysin (MMP-7), but the factors regulating this expression have not been identified. Fibroblast growth factors (FGF); which are expressed in the prostate, might participate in paracrine regulation of matrilysin expression by prostate cancer cells. METHODS. We tested the ability of recombinant FGF proteins and prostate fibroblast-conditioned media (PFCM) to induce promatrilysin expression in the prostate carcinoma cell line, LNCaP, and in normal prostate epithelial (PrEC) cells. We also characterized prostate fibroblast FGF expression by reverse transcriptase-polymerase chain reaction (RT-PCR). An inhibitor of FGF receptor activation (SU5402) was used to determine the role of FGF proteins in the induction of promatrilysin expression by PFCM. RESULTS. Recombinant FGF-1, FGF-2, FGF-9, FGF-10, and PFCM significantly induced promatrilysin expression in LNCaP cells but not in PrEC cells. Prostate fibroblasts express mRNAs for these FGF proteins, and inhibition of LNCaP cell FGF receptors with SU5402 substantially reduced the induction of promatrilysin expression by PFCM. CONCLUSIONS. Stromally expressed FGF proteins induce promatrilysin expression in a prostate carcinoma cell, and may provide a mechanism for the overexpression of promatrilysin observed in prostate cancer.

AB - BACKGROUND. It has been determined that prostate cancer cells overexpress the matrix metalloprotease matrilysin (MMP-7), but the factors regulating this expression have not been identified. Fibroblast growth factors (FGF); which are expressed in the prostate, might participate in paracrine regulation of matrilysin expression by prostate cancer cells. METHODS. We tested the ability of recombinant FGF proteins and prostate fibroblast-conditioned media (PFCM) to induce promatrilysin expression in the prostate carcinoma cell line, LNCaP, and in normal prostate epithelial (PrEC) cells. We also characterized prostate fibroblast FGF expression by reverse transcriptase-polymerase chain reaction (RT-PCR). An inhibitor of FGF receptor activation (SU5402) was used to determine the role of FGF proteins in the induction of promatrilysin expression by PFCM. RESULTS. Recombinant FGF-1, FGF-2, FGF-9, FGF-10, and PFCM significantly induced promatrilysin expression in LNCaP cells but not in PrEC cells. Prostate fibroblasts express mRNAs for these FGF proteins, and inhibition of LNCaP cell FGF receptors with SU5402 substantially reduced the induction of promatrilysin expression by PFCM. CONCLUSIONS. Stromally expressed FGF proteins induce promatrilysin expression in a prostate carcinoma cell, and may provide a mechanism for the overexpression of promatrilysin observed in prostate cancer.

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