Abstract
The two cytochromes (cyt) b558 of acidic nature, one-95-100 kDa and another one, 60-70 kDa were isolated for the first time from the human's lymphosarcoma tissue cells using gel filtration and ion exchange chromatography. These hemoproteins possess NADPH dependent O2 --producing and ferrihemoglobin-reducing activities. The incubation of neuropeptide PRP-1 (5 μg) with cytochrome b558, caused elevation of these activities. The gel filtration results indicated possible binding of PRP-1 to these cytochromes b558. PRP-1 activated both NADPH dependent O2 --producing and ferriHb-reducing activities of the cyt b1 558 and cyt b2 558, obtained from human lymphosarcoma tissue cells. One can assume that PRP-1 associated with cyt b 558 on the surface of the tumor cells by increasing both NADPH dependent O2 --producing and ferriHb-reducing activities of cyt b558, increases the oxidation- reduction status. Changing the oxidation-reduction status and oxygen homeostasis of the tumor cells by PRP-1 can serve as one of the possible explanation of antitumorigenic effect of this cytokine.
| Original language | English |
|---|---|
| Pages (from-to) | 739-745 |
| Number of pages | 7 |
| Journal | Neurochemical Research |
| Volume | 36 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 1 2011 |
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Keywords
- Cytochrome b
- Human lymphosarcoma
- Metalloproteins
- NADPH oxidase
- Prolin rich polypeptide
- Reactive oxygen species
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Biochemistry
Cite this
Proline rich polypeptide (prp-1) increases the superoxide-producing and ferrihemoglobin reducing activities of cytochrome b558 isoforms from human lymphosarcoma tissue cells. / Simonyan, G. M.; Galoian, Karina; Simonyan, R. M.; Simonyan, M. A.; Galoyan, A. A.
In: Neurochemical Research, Vol. 36, No. 5, 01.05.2011, p. 739-745.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proline rich polypeptide (prp-1) increases the superoxide-producing and ferrihemoglobin reducing activities of cytochrome b558 isoforms from human lymphosarcoma tissue cells
AU - Simonyan, G. M.
AU - Galoian, Karina
AU - Simonyan, R. M.
AU - Simonyan, M. A.
AU - Galoyan, A. A.
PY - 2011/5/1
Y1 - 2011/5/1
N2 - The two cytochromes (cyt) b558 of acidic nature, one-95-100 kDa and another one, 60-70 kDa were isolated for the first time from the human's lymphosarcoma tissue cells using gel filtration and ion exchange chromatography. These hemoproteins possess NADPH dependent O2 --producing and ferrihemoglobin-reducing activities. The incubation of neuropeptide PRP-1 (5 μg) with cytochrome b558, caused elevation of these activities. The gel filtration results indicated possible binding of PRP-1 to these cytochromes b558. PRP-1 activated both NADPH dependent O2 --producing and ferriHb-reducing activities of the cyt b1 558 and cyt b2 558, obtained from human lymphosarcoma tissue cells. One can assume that PRP-1 associated with cyt b 558 on the surface of the tumor cells by increasing both NADPH dependent O2 --producing and ferriHb-reducing activities of cyt b558, increases the oxidation- reduction status. Changing the oxidation-reduction status and oxygen homeostasis of the tumor cells by PRP-1 can serve as one of the possible explanation of antitumorigenic effect of this cytokine.
AB - The two cytochromes (cyt) b558 of acidic nature, one-95-100 kDa and another one, 60-70 kDa were isolated for the first time from the human's lymphosarcoma tissue cells using gel filtration and ion exchange chromatography. These hemoproteins possess NADPH dependent O2 --producing and ferrihemoglobin-reducing activities. The incubation of neuropeptide PRP-1 (5 μg) with cytochrome b558, caused elevation of these activities. The gel filtration results indicated possible binding of PRP-1 to these cytochromes b558. PRP-1 activated both NADPH dependent O2 --producing and ferriHb-reducing activities of the cyt b1 558 and cyt b2 558, obtained from human lymphosarcoma tissue cells. One can assume that PRP-1 associated with cyt b 558 on the surface of the tumor cells by increasing both NADPH dependent O2 --producing and ferriHb-reducing activities of cyt b558, increases the oxidation- reduction status. Changing the oxidation-reduction status and oxygen homeostasis of the tumor cells by PRP-1 can serve as one of the possible explanation of antitumorigenic effect of this cytokine.
KW - Cytochrome b
KW - Human lymphosarcoma
KW - Metalloproteins
KW - NADPH oxidase
KW - Prolin rich polypeptide
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=79954445576&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79954445576&partnerID=8YFLogxK
U2 - 10.1007/s11064-010-0389-7
DO - 10.1007/s11064-010-0389-7
M3 - Article
C2 - 21213045
AN - SCOPUS:79954445576
VL - 36
SP - 739
EP - 745
JO - Neurochemical Research
JF - Neurochemical Research
SN - 0364-3190
IS - 5
ER -