Proline rich polypeptide (prp-1) increases the superoxide-producing and ferrihemoglobin reducing activities of cytochrome b558 isoforms from human lymphosarcoma tissue cells

G. M. Simonyan, Karina Galoian, R. M. Simonyan, M. A. Simonyan, A. A. Galoyan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The two cytochromes (cyt) b558 of acidic nature, one-95-100 kDa and another one, 60-70 kDa were isolated for the first time from the human's lymphosarcoma tissue cells using gel filtration and ion exchange chromatography. These hemoproteins possess NADPH dependent O2 --producing and ferrihemoglobin-reducing activities. The incubation of neuropeptide PRP-1 (5 μg) with cytochrome b558, caused elevation of these activities. The gel filtration results indicated possible binding of PRP-1 to these cytochromes b558. PRP-1 activated both NADPH dependent O2 --producing and ferriHb-reducing activities of the cyt b1 558 and cyt b2 558, obtained from human lymphosarcoma tissue cells. One can assume that PRP-1 associated with cyt b 558 on the surface of the tumor cells by increasing both NADPH dependent O2 --producing and ferriHb-reducing activities of cyt b558, increases the oxidation- reduction status. Changing the oxidation-reduction status and oxygen homeostasis of the tumor cells by PRP-1 can serve as one of the possible explanation of antitumorigenic effect of this cytokine.

Original languageEnglish
Pages (from-to)739-745
Number of pages7
JournalNeurochemical Research
Volume36
Issue number5
DOIs
StatePublished - May 1 2011

Fingerprint

PRP-1 peptide
Methemoglobin
Proline
Superoxides
Non-Hodgkin's Lymphoma
Protein Isoforms
Tissue
Peptides
NADP
Oxidation-Reduction
Gel Chromatography
Tumors
L-Lactate Dehydrogenase (Cytochrome)
Gels
Cells
Ion Exchange Chromatography
Chromatography
Neuropeptides
Neoplasms
Ion exchange

Keywords

  • Cytochrome b
  • Human lymphosarcoma
  • Metalloproteins
  • NADPH oxidase
  • Prolin rich polypeptide
  • Reactive oxygen species

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Biochemistry

Cite this

Proline rich polypeptide (prp-1) increases the superoxide-producing and ferrihemoglobin reducing activities of cytochrome b558 isoforms from human lymphosarcoma tissue cells. / Simonyan, G. M.; Galoian, Karina; Simonyan, R. M.; Simonyan, M. A.; Galoyan, A. A.

In: Neurochemical Research, Vol. 36, No. 5, 01.05.2011, p. 739-745.

Research output: Contribution to journalArticle

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