Abstract
We have tested the dividing cells in the mouse thymus for expression of interleukin 2 (IL-2) receptors (IL-2-R) using the rat monoclonal antibody 7D4. A discrete subpopulation of the lymphoblasts clearly expressed IL-2-R at levels comparable to those on mitogen-activated peripheral T cells. This subpopulation, however, represented a small minority of the proliferating cells. IL-2-R-bearing cells were depleted from the PNA+ (peanut agglutinin) lymphoblast population, which contains the direct precursors of most of the cells in the thymus. The majority of receptor-bearing cells were found in the PNA- lymphoblast population, where they constituted only ~12% of the cells. Thus, virtually all the PNA+ and most of the PNA- blast cells were in cycle without detectable IL-2-R expression. This indicates that they were not dividing in response to IL-2, and implies that they were not dividing in response to antigen, but rather to novel thymus-specific mitogenic stimuli. On the other hand, the proliferating cells that do express IL-2-R were enriched 4-5-fold in the rapidly growing neonatal thymus, suggesting that they may also play a key role in T cell development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1048-1062 |
| Number of pages | 15 |
| Journal | Journal of Experimental Medicine |
| Volume | 161 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Proliferation of thymic stem cells with and without receptors for interleukin 2. Implications for intrathymic antigen recognition. / Lugo, J. P.; Krishnan, S. N.; Sailor, R. D.; Koen, P.; Malek, Thomas; Rothenberg, E.
In: Journal of Experimental Medicine, Vol. 161, No. 5, 1985, p. 1048-1062.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proliferation of thymic stem cells with and without receptors for interleukin 2. Implications for intrathymic antigen recognition
AU - Lugo, J. P.
AU - Krishnan, S. N.
AU - Sailor, R. D.
AU - Koen, P.
AU - Malek, Thomas
AU - Rothenberg, E.
PY - 1985
Y1 - 1985
N2 - We have tested the dividing cells in the mouse thymus for expression of interleukin 2 (IL-2) receptors (IL-2-R) using the rat monoclonal antibody 7D4. A discrete subpopulation of the lymphoblasts clearly expressed IL-2-R at levels comparable to those on mitogen-activated peripheral T cells. This subpopulation, however, represented a small minority of the proliferating cells. IL-2-R-bearing cells were depleted from the PNA+ (peanut agglutinin) lymphoblast population, which contains the direct precursors of most of the cells in the thymus. The majority of receptor-bearing cells were found in the PNA- lymphoblast population, where they constituted only ~12% of the cells. Thus, virtually all the PNA+ and most of the PNA- blast cells were in cycle without detectable IL-2-R expression. This indicates that they were not dividing in response to IL-2, and implies that they were not dividing in response to antigen, but rather to novel thymus-specific mitogenic stimuli. On the other hand, the proliferating cells that do express IL-2-R were enriched 4-5-fold in the rapidly growing neonatal thymus, suggesting that they may also play a key role in T cell development.
AB - We have tested the dividing cells in the mouse thymus for expression of interleukin 2 (IL-2) receptors (IL-2-R) using the rat monoclonal antibody 7D4. A discrete subpopulation of the lymphoblasts clearly expressed IL-2-R at levels comparable to those on mitogen-activated peripheral T cells. This subpopulation, however, represented a small minority of the proliferating cells. IL-2-R-bearing cells were depleted from the PNA+ (peanut agglutinin) lymphoblast population, which contains the direct precursors of most of the cells in the thymus. The majority of receptor-bearing cells were found in the PNA- lymphoblast population, where they constituted only ~12% of the cells. Thus, virtually all the PNA+ and most of the PNA- blast cells were in cycle without detectable IL-2-R expression. This indicates that they were not dividing in response to IL-2, and implies that they were not dividing in response to antigen, but rather to novel thymus-specific mitogenic stimuli. On the other hand, the proliferating cells that do express IL-2-R were enriched 4-5-fold in the rapidly growing neonatal thymus, suggesting that they may also play a key role in T cell development.
UR - http://www.scopus.com/inward/record.url?scp=0021876317&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021876317&partnerID=8YFLogxK
U2 - 10.1084/jem.161.5.1048
DO - 10.1084/jem.161.5.1048
M3 - Article
C2 - 3921650
AN - SCOPUS:0021876317
VL - 161
SP - 1048
EP - 1062
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 5
ER -