Proliferation kinetics of recruited cells in a mouse mammary carcinoma

Alan Pollack, Nicholas H A Terry, R. Allen White, Shilong Cao, Marvin L. Meistrich, Luka Milas

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Solid tumors contain populations of proliferating (P) and quiescent (Q) cells. Shifting between these populations occurs continuously and cells are recruited from quiescence to proliferate (Q→P) as a result of exogenously applied or endogenous cell depleting stimuli. Direct measurements of the proliferation kinetics of these Q→P cells in solid tumors are difficult to make because of the much larger percentage of P-cells. In order to specifically analyze the kinetics of the Q→P cells, double thymidine analogue labeling was used. This was accomplished by first labeling in vivo all of the P-cells in MCaK tumors using continuous exposure to chlorodeoxyuridine (CldUrd) administered by a minipump over 21 h. About 75% of the aneuploid cells are P-cells based on CldUrd labeling. At different times after the pumps were removed, the tumors were pulse-labeled with iododeoxyuridine (IdUrd) and harvested 6 h later. A 3-color flow cytometry assay was used to simultaneously and independently analyze CldUrd and IdUrd incorporation, as well as DNA content. The Q→P cells were identified as having only been labeled with IdUrd. The length of their S-phase was calculated from the movement of the Q→P cells during the 6 h after IdUrd labeling. The results showed the length of S-phase for the recruited cells to be slightly, but significantly, longer than the length of S-phase for the total cells (11 h versus 9 h, respectively). Thus, the recruited cells appear to have slightly slower kinetics than the proliferating cells in the absence of a perturbing stimulus such as radiotherapy or chemotherapy.

Original languageEnglish
Pages (from-to)811-817
Number of pages7
JournalCancer Research
Volume54
Issue number3
StatePublished - Feb 1 1994
Externally publishedYes

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Breast Neoplasms
Idoxuridine
S Phase
Neoplasms
Aneuploidy
Thymidine
Population
Flow Cytometry
Radiotherapy
Color
Drug Therapy
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Pollack, A., Terry, N. H. A., White, R. A., Cao, S., Meistrich, M. L., & Milas, L. (1994). Proliferation kinetics of recruited cells in a mouse mammary carcinoma. Cancer Research, 54(3), 811-817.

Proliferation kinetics of recruited cells in a mouse mammary carcinoma. / Pollack, Alan; Terry, Nicholas H A; White, R. Allen; Cao, Shilong; Meistrich, Marvin L.; Milas, Luka.

In: Cancer Research, Vol. 54, No. 3, 01.02.1994, p. 811-817.

Research output: Contribution to journalArticle

Pollack, A, Terry, NHA, White, RA, Cao, S, Meistrich, ML & Milas, L 1994, 'Proliferation kinetics of recruited cells in a mouse mammary carcinoma', Cancer Research, vol. 54, no. 3, pp. 811-817.
Pollack A, Terry NHA, White RA, Cao S, Meistrich ML, Milas L. Proliferation kinetics of recruited cells in a mouse mammary carcinoma. Cancer Research. 1994 Feb 1;54(3):811-817.
Pollack, Alan ; Terry, Nicholas H A ; White, R. Allen ; Cao, Shilong ; Meistrich, Marvin L. ; Milas, Luka. / Proliferation kinetics of recruited cells in a mouse mammary carcinoma. In: Cancer Research. 1994 ; Vol. 54, No. 3. pp. 811-817.
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