Proliferation and neuronal differentiation of mitotically active cells following traumatic brain injury

A. C. Rice, A. Khaldi, H. B. Harvey, N. J. Salman, F. White, H. Fillmore, Ross Bullock

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

Recent studies have identified endogenous neural stem cells in adult rodent brains. The present study characterizes the early response of mitotically active cells in the brain to traumatic brain injury. Animals were subjected to lateral fluid percussion injury and sacrificed at various times after injury. To examine increases in cell proliferation animals were injected with the mitotic marker bromodeoxyuridine (BrdU) 24 h before sacrifice. Increased numbers of mitotically active cells were observed at 2 days in the subgranular zone (SGZ) and the subependymal zone (SEZ) under the injury site. To characterize the differentiation potential of these cells, animals were injected with BrdU 18 and 20 h after injury, then sacrificed at multiple time points after injury. Histologically, co-localization with βIII-tubulin (neuronal marker) and BrdU was evident at 10 and 15 days postinjury in the SGZ. Flow cytometry analysis was used to quantitatively assess neurogenesis in the SEZ. Animals were sacrificed 1, 5, or 10 days after injury and tissue sections extracted, grown in tissue culture for 24 h, fixed, and stained for nestin and βIII-tubulin to identify newly formed neurons. The percentage of cells expressing both markers was determined using flow cytometry analysis. There was a significant increase in newly differentiated neurons by 10 days postinjury in the SEZ. Thus, we conclude that traumatic brain injury stimulates an increase in proliferation of endogenous neural stem/progenitor cells and that a significant number of these express a neuronal marker. This response may be the brain's way of trying to heal itself after injury.

Original languageEnglish
Pages (from-to)406-417
Number of pages12
JournalExperimental Neurology
Volume183
Issue number2
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

Wounds and Injuries
Bromodeoxyuridine
Neural Stem Cells
Tubulin
Flow Cytometry
Brain
Percussion
Neurons
Nestin
Neurogenesis
Traumatic Brain Injury
Cell Differentiation
Rodentia
Stem Cells
Cell Proliferation

Keywords

  • Bromodeoxyuridine
  • Confocal microscopy
  • Flow cytometry
  • Lateral fluid percussion injury
  • Neurogenesis

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

Rice, A. C., Khaldi, A., Harvey, H. B., Salman, N. J., White, F., Fillmore, H., & Bullock, R. (2003). Proliferation and neuronal differentiation of mitotically active cells following traumatic brain injury. Experimental Neurology, 183(2), 406-417. https://doi.org/10.1016/S0014-4886(03)00241-3

Proliferation and neuronal differentiation of mitotically active cells following traumatic brain injury. / Rice, A. C.; Khaldi, A.; Harvey, H. B.; Salman, N. J.; White, F.; Fillmore, H.; Bullock, Ross.

In: Experimental Neurology, Vol. 183, No. 2, 01.10.2003, p. 406-417.

Research output: Contribution to journalArticle

Rice, AC, Khaldi, A, Harvey, HB, Salman, NJ, White, F, Fillmore, H & Bullock, R 2003, 'Proliferation and neuronal differentiation of mitotically active cells following traumatic brain injury', Experimental Neurology, vol. 183, no. 2, pp. 406-417. https://doi.org/10.1016/S0014-4886(03)00241-3
Rice, A. C. ; Khaldi, A. ; Harvey, H. B. ; Salman, N. J. ; White, F. ; Fillmore, H. ; Bullock, Ross. / Proliferation and neuronal differentiation of mitotically active cells following traumatic brain injury. In: Experimental Neurology. 2003 ; Vol. 183, No. 2. pp. 406-417.
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