Progressive and reproducible focal cortical ischemia with or without late spontaneous reperfusion generated by a ring-shaped, laser-driven photothrombotic lesion in rats

Xiao Lei Hu, Per Wester, Thomas Brännström, Brant D. Watson, Wei Gang Gu

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Clinical stroke is mostly of thromboembolic origin, in which the magnitude of brain damage resulting from arterial occlusions depends on the degree and duration of the concomitant ischemia. To facilitate more controllable and reproducible study of stroke-related pathophysiological mechanisms, a photothrombotic ring stroke model was initially developed in adult rats. The ring interior zone comprises an anatomically well confined cortical region-at-risk which is gradually encroached by progressive hypoperfusion, thus mimicking the situation (albeit in inverse fashion) of an ischemic penumbra or stroke-in-evolution. Modification of this model using a thinner ring irradiation beam resulted in late spontaneous reperfusion in the cortical region-at-risk and a remarkable morphological tissue recovery in this ostensibly critically injured region. On the other hand, doubling the thin irradiating beam intensity facilitates a complementary situation in which lack of reperfusion in the region-at-risk after stroke induction leads to tissue pannecrosis. The dual photothrombotic ring stroke model, effectuated either with or without reperfusion and thereby tissue recovery or pannecrosis, may be well suited for the study of events related to postischemic survival or cell death in the penumbra region. To popularize the photothrombotic ring stroke model, we present a detailed protocol of how this model is induced in either version as well as protocols for transcardial carbon black perfusion and laser-Doppler flowmetry experiments.

Original languageEnglish (US)
Pages (from-to)76-85
Number of pages10
JournalBrain Research Protocols
Volume7
Issue number1
DOIs
StatePublished - 2001

Keywords

  • Cerebral ischemia, focal
  • Laser-Doppler flowmetry
  • Penumbra
  • Photothrombosis
  • Rat
  • Reperfusion

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Progressive and reproducible focal cortical ischemia with or without late spontaneous reperfusion generated by a ring-shaped, laser-driven photothrombotic lesion in rats'. Together they form a unique fingerprint.

  • Cite this