Prognostic role of p-27, a cyclin dependent kinase inhibitor (cdk-i) in localized prostate cancer

John Tsihlias, Linda Kapusta, Joyce M. Slingerland, G. Charles Catzavelos, G. Deboer, Laurence H. Klotz

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: p27 is a cyclin dependent kinase inhibitor which negatively regulates cell proliferation by mediating cell cycle arrest in the Gl phase. In primary breast and colon adenocarcinomas reduced p27 protein correlates with a more malignant phenotype and poor disease outcome for the patient. The present study was undertaken to assess the prognostic value of p27 in primary prostate cancer. Methods: Archival paraffin embedded tissue was retrieved form radical prostatectomy specimens recovered between 1985 and 1993 (n=129). p27 protein was stained immunohistochemically using a commercially available monoclonal Ab for p27. Normal prostate tissue adjacent to the cancer served as an internal control. Patient charts were reviewed for pre-operative PSA, clinical and pathological staging, Gleason Sum, time to biochemical and clinical recurrence, and survival. Results: Strong p27 staining was uniformly seen in benign prostatic epithelial components in all tumour sections. p27 staining was reduced in most prostate cancers and in PIN. A subset exhibited marked decrease in p27 staining. In the intermediate grade cancers (Gleason 6-7) this heterogeneity was preserved; some of these cancers stained strongly, whereas others demonstrated weak or absent staining for p27. Decreased p27 staining correlated positively with higher Gleason sum. A correlation with time to progression was seen. Conclusions: These findings raise the intriguing possibility that loss of p27 expression may offer prognostic information independent of Gleason grading in localized prostate cancer. The results of the multivariate analysis of p27 as a prognostic factor will be presented.

Original languageEnglish (US)
Number of pages1
JournalBritish Journal of Urology
Volume80
Issue numberSUPPL. 2
StatePublished - Dec 1 1997
Externally publishedYes

ASJC Scopus subject areas

  • Urology

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