Prognostic parameters in uveal melanoma and their association with bap1 expression

T. Huibertus Van Essen, Sake Van Pelt, Mieke Versluis, Inge H G Bronkhorst, Sjoerd G. Van Duinen, Marina Marinkovic, Wilma G M Kroes, Claudia A L Ruivenkamp, Shruti Shukla, Annelies De Klein, Emine Kiliç, J. William Harbour, Gregorius P M Luyten, Pieter A. Van Der Velden, Rob M. Verdijk, Martine J. Jager

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Methods Thirty cases of uveal melanoma obtained by enucleation between 1999 and 2004 were analysed for a variety of prognostic markers, including histological characteristics, chromosome aberrations obtained by fluorescence in situ hybridisation (FISH) and single nucleotide polymorphism (SNP) analysis and gene expression profiling. These parameters were compared with BAP1 gene expression and BAP1 immunostaining.

Results The presence of monosomy of chromosome 3 as identified by the different chromosome 3 tests showed significantly increased HRs (FISH on isolated nuclei cut-off 30%: HR 11.6, p=0.002; SNP analysis: HR 20.3, p=0.004) for death due to metastasis. The gene expression profile class 2, based on the 15-gene expression profile, similarly provided a significantly increased HR for a poor outcome (HR 8.5, p=0.005). Lower BAP1 gene expression and negative BAP1 immunostaining (50% of 28 tumours were immunonegative) were both associated with these markers for prognostication: FISH cut-off 30% monosomy 3 (BAP1 gene expression: p=0.037; BAP1 immunostaining: p=0.001), SNP-monosomy 3 (BAP1 gene expression: p=0.008; BAP1 immunostaining: p=0.002) and class 2 profile (BAP1 gene expression: p<0.001; BAP1 immunostaining: p=0.001) and were themselves associated with an increased risk of death due to metastasis (BAP1 gene expression dichotomised: HR 8.7, p=0.006; BAP1 immunostaining: HR 4.0, p=0.010).

Conclusions Loss of BAP1 expression associated well with all of the methods currently used for prognostication and was itself predictive of death due to metastasis in uveal melanoma after enucleation, thereby emphasising the importance of further research on the role of BAP1 in uveal melanoma. .

Aim To determine whether BAP1 gene and protein expression associates with different prognostic parameters in uveal melanoma and whether BAP1 expression correctly identi fies patients as being at risk for metastases, following enucleation of the primary tumour.

Original languageEnglish
Pages (from-to)1738-1743
Number of pages6
JournalBritish Journal of Ophthalmology
Volume98
Issue number12
DOIs
StatePublished - Jan 1 2014

Fingerprint

Gene Expression
Monosomy
Fluorescence In Situ Hybridization
Transcriptome
Neoplasm Metastasis
Single Nucleotide Polymorphism
Chromosomes, Human, Pair 3
Gene Expression Profiling
Chromosome Aberrations
Uveal melanoma
Neoplasms
Research
Proteins

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Van Essen, T. H., Van Pelt, S., Versluis, M., Bronkhorst, I. H. G., Van Duinen, S. G., Marinkovic, M., ... Jager, M. J. (2014). Prognostic parameters in uveal melanoma and their association with bap1 expression. British Journal of Ophthalmology, 98(12), 1738-1743. https://doi.org/10.1136/bjophthalmol-2014-305047

Prognostic parameters in uveal melanoma and their association with bap1 expression. / Van Essen, T. Huibertus; Van Pelt, Sake; Versluis, Mieke; Bronkhorst, Inge H G; Van Duinen, Sjoerd G.; Marinkovic, Marina; Kroes, Wilma G M; Ruivenkamp, Claudia A L; Shukla, Shruti; De Klein, Annelies; Kiliç, Emine; William Harbour, J.; Luyten, Gregorius P M; Van Der Velden, Pieter A.; Verdijk, Rob M.; Jager, Martine J.

In: British Journal of Ophthalmology, Vol. 98, No. 12, 01.01.2014, p. 1738-1743.

Research output: Contribution to journalArticle

Van Essen, TH, Van Pelt, S, Versluis, M, Bronkhorst, IHG, Van Duinen, SG, Marinkovic, M, Kroes, WGM, Ruivenkamp, CAL, Shukla, S, De Klein, A, Kiliç, E, William Harbour, J, Luyten, GPM, Van Der Velden, PA, Verdijk, RM & Jager, MJ 2014, 'Prognostic parameters in uveal melanoma and their association with bap1 expression', British Journal of Ophthalmology, vol. 98, no. 12, pp. 1738-1743. https://doi.org/10.1136/bjophthalmol-2014-305047
Van Essen TH, Van Pelt S, Versluis M, Bronkhorst IHG, Van Duinen SG, Marinkovic M et al. Prognostic parameters in uveal melanoma and their association with bap1 expression. British Journal of Ophthalmology. 2014 Jan 1;98(12):1738-1743. https://doi.org/10.1136/bjophthalmol-2014-305047
Van Essen, T. Huibertus ; Van Pelt, Sake ; Versluis, Mieke ; Bronkhorst, Inge H G ; Van Duinen, Sjoerd G. ; Marinkovic, Marina ; Kroes, Wilma G M ; Ruivenkamp, Claudia A L ; Shukla, Shruti ; De Klein, Annelies ; Kiliç, Emine ; William Harbour, J. ; Luyten, Gregorius P M ; Van Der Velden, Pieter A. ; Verdijk, Rob M. ; Jager, Martine J. / Prognostic parameters in uveal melanoma and their association with bap1 expression. In: British Journal of Ophthalmology. 2014 ; Vol. 98, No. 12. pp. 1738-1743.
@article{2584e34020e049eeae5a0e4fce2634a2,
title = "Prognostic parameters in uveal melanoma and their association with bap1 expression",
abstract = "Methods Thirty cases of uveal melanoma obtained by enucleation between 1999 and 2004 were analysed for a variety of prognostic markers, including histological characteristics, chromosome aberrations obtained by fluorescence in situ hybridisation (FISH) and single nucleotide polymorphism (SNP) analysis and gene expression profiling. These parameters were compared with BAP1 gene expression and BAP1 immunostaining.Results The presence of monosomy of chromosome 3 as identified by the different chromosome 3 tests showed significantly increased HRs (FISH on isolated nuclei cut-off 30{\%}: HR 11.6, p=0.002; SNP analysis: HR 20.3, p=0.004) for death due to metastasis. The gene expression profile class 2, based on the 15-gene expression profile, similarly provided a significantly increased HR for a poor outcome (HR 8.5, p=0.005). Lower BAP1 gene expression and negative BAP1 immunostaining (50{\%} of 28 tumours were immunonegative) were both associated with these markers for prognostication: FISH cut-off 30{\%} monosomy 3 (BAP1 gene expression: p=0.037; BAP1 immunostaining: p=0.001), SNP-monosomy 3 (BAP1 gene expression: p=0.008; BAP1 immunostaining: p=0.002) and class 2 profile (BAP1 gene expression: p<0.001; BAP1 immunostaining: p=0.001) and were themselves associated with an increased risk of death due to metastasis (BAP1 gene expression dichotomised: HR 8.7, p=0.006; BAP1 immunostaining: HR 4.0, p=0.010).Conclusions Loss of BAP1 expression associated well with all of the methods currently used for prognostication and was itself predictive of death due to metastasis in uveal melanoma after enucleation, thereby emphasising the importance of further research on the role of BAP1 in uveal melanoma. .Aim To determine whether BAP1 gene and protein expression associates with different prognostic parameters in uveal melanoma and whether BAP1 expression correctly identi fies patients as being at risk for metastases, following enucleation of the primary tumour.",
author = "{Van Essen}, {T. Huibertus} and {Van Pelt}, Sake and Mieke Versluis and Bronkhorst, {Inge H G} and {Van Duinen}, {Sjoerd G.} and Marina Marinkovic and Kroes, {Wilma G M} and Ruivenkamp, {Claudia A L} and Shruti Shukla and {De Klein}, Annelies and Emine Kili{\cc} and {William Harbour}, J. and Luyten, {Gregorius P M} and {Van Der Velden}, {Pieter A.} and Verdijk, {Rob M.} and Jager, {Martine J.}",
year = "2014",
month = "1",
day = "1",
doi = "10.1136/bjophthalmol-2014-305047",
language = "English",
volume = "98",
pages = "1738--1743",
journal = "British Journal of Ophthalmology",
issn = "0007-1161",
publisher = "BMJ Publishing Group",
number = "12",

}

TY - JOUR

T1 - Prognostic parameters in uveal melanoma and their association with bap1 expression

AU - Van Essen, T. Huibertus

AU - Van Pelt, Sake

AU - Versluis, Mieke

AU - Bronkhorst, Inge H G

AU - Van Duinen, Sjoerd G.

AU - Marinkovic, Marina

AU - Kroes, Wilma G M

AU - Ruivenkamp, Claudia A L

AU - Shukla, Shruti

AU - De Klein, Annelies

AU - Kiliç, Emine

AU - William Harbour, J.

AU - Luyten, Gregorius P M

AU - Van Der Velden, Pieter A.

AU - Verdijk, Rob M.

AU - Jager, Martine J.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Methods Thirty cases of uveal melanoma obtained by enucleation between 1999 and 2004 were analysed for a variety of prognostic markers, including histological characteristics, chromosome aberrations obtained by fluorescence in situ hybridisation (FISH) and single nucleotide polymorphism (SNP) analysis and gene expression profiling. These parameters were compared with BAP1 gene expression and BAP1 immunostaining.Results The presence of monosomy of chromosome 3 as identified by the different chromosome 3 tests showed significantly increased HRs (FISH on isolated nuclei cut-off 30%: HR 11.6, p=0.002; SNP analysis: HR 20.3, p=0.004) for death due to metastasis. The gene expression profile class 2, based on the 15-gene expression profile, similarly provided a significantly increased HR for a poor outcome (HR 8.5, p=0.005). Lower BAP1 gene expression and negative BAP1 immunostaining (50% of 28 tumours were immunonegative) were both associated with these markers for prognostication: FISH cut-off 30% monosomy 3 (BAP1 gene expression: p=0.037; BAP1 immunostaining: p=0.001), SNP-monosomy 3 (BAP1 gene expression: p=0.008; BAP1 immunostaining: p=0.002) and class 2 profile (BAP1 gene expression: p<0.001; BAP1 immunostaining: p=0.001) and were themselves associated with an increased risk of death due to metastasis (BAP1 gene expression dichotomised: HR 8.7, p=0.006; BAP1 immunostaining: HR 4.0, p=0.010).Conclusions Loss of BAP1 expression associated well with all of the methods currently used for prognostication and was itself predictive of death due to metastasis in uveal melanoma after enucleation, thereby emphasising the importance of further research on the role of BAP1 in uveal melanoma. .Aim To determine whether BAP1 gene and protein expression associates with different prognostic parameters in uveal melanoma and whether BAP1 expression correctly identi fies patients as being at risk for metastases, following enucleation of the primary tumour.

AB - Methods Thirty cases of uveal melanoma obtained by enucleation between 1999 and 2004 were analysed for a variety of prognostic markers, including histological characteristics, chromosome aberrations obtained by fluorescence in situ hybridisation (FISH) and single nucleotide polymorphism (SNP) analysis and gene expression profiling. These parameters were compared with BAP1 gene expression and BAP1 immunostaining.Results The presence of monosomy of chromosome 3 as identified by the different chromosome 3 tests showed significantly increased HRs (FISH on isolated nuclei cut-off 30%: HR 11.6, p=0.002; SNP analysis: HR 20.3, p=0.004) for death due to metastasis. The gene expression profile class 2, based on the 15-gene expression profile, similarly provided a significantly increased HR for a poor outcome (HR 8.5, p=0.005). Lower BAP1 gene expression and negative BAP1 immunostaining (50% of 28 tumours were immunonegative) were both associated with these markers for prognostication: FISH cut-off 30% monosomy 3 (BAP1 gene expression: p=0.037; BAP1 immunostaining: p=0.001), SNP-monosomy 3 (BAP1 gene expression: p=0.008; BAP1 immunostaining: p=0.002) and class 2 profile (BAP1 gene expression: p<0.001; BAP1 immunostaining: p=0.001) and were themselves associated with an increased risk of death due to metastasis (BAP1 gene expression dichotomised: HR 8.7, p=0.006; BAP1 immunostaining: HR 4.0, p=0.010).Conclusions Loss of BAP1 expression associated well with all of the methods currently used for prognostication and was itself predictive of death due to metastasis in uveal melanoma after enucleation, thereby emphasising the importance of further research on the role of BAP1 in uveal melanoma. .Aim To determine whether BAP1 gene and protein expression associates with different prognostic parameters in uveal melanoma and whether BAP1 expression correctly identi fies patients as being at risk for metastases, following enucleation of the primary tumour.

UR - http://www.scopus.com/inward/record.url?scp=84911451920&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84911451920&partnerID=8YFLogxK

U2 - 10.1136/bjophthalmol-2014-305047

DO - 10.1136/bjophthalmol-2014-305047

M3 - Article

C2 - 25147369

AN - SCOPUS:84911451920

VL - 98

SP - 1738

EP - 1743

JO - British Journal of Ophthalmology

JF - British Journal of Ophthalmology

SN - 0007-1161

IS - 12

ER -