Progesterone directly and indirectly affects perforin expression in cytolytic cells

G. Laškarin, Zs Faust, Natasa Strbo, V. Sotošek, J. Szekeres-Bartho, E. R. Podack, Daniel Rukavina

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

PROBLEM: Decidual lymphocytes (DL) expressing the cytolytic molecule perforin represent approximately 55% of DL in the first trimester of human pregnancy. Progesterone dominates this phase of pregnancy and controls the production of uterine cytokines and growth factors. The aim of this study was to investigate the role of progesterone and progesterone-induced blocking factor (PIBF) on perforin expression in DL and peripheral blood lymphocytes (PBL). METHOD OF STUDY: Perforin expression was analyzed in PBL and DL incubated either in culture medium or with decidual adherent cells (DAC) and peripheral blood adherent cells (PBAC) and their supernatants with or without progesterone or PIBF. Perforin was detected by flow cytometry in PB and in decidual first trimester pregnancy lymphocytes. RESULTS: Progesterone in high concentrations directly affects perforin expression in DL but not in PBL. Progesterone in a concentration dependent manner indirectly blocks perforin expression in DL and PBL cultured with adherent cells or their supernatants. PIBF blocked upregulation of perforin expression of DL cultured with DAC, but none of those cultured with PBAC. Similarly, PIBF was inefficient when PBL or DL were cultured with PBAC. CONCLUSION: Progesterone present in a high concentration locally at the maternal-fetal interface modulates perforin expression in the first trimester pregnancy DL.

Original languageEnglish
Pages (from-to)312-320
Number of pages9
JournalAmerican Journal of Reproductive Immunology
Volume42
Issue number5
StatePublished - Nov 1 1999
Externally publishedYes

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Perforin
Progesterone
Lymphocytes
First Pregnancy Trimester
Blood Cells

Keywords

  • Decidual granular lymphocytes
  • First trimester pregnancy
  • Perforin
  • Peripheral blood lymphocytes
  • PIBF
  • Progesterone

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Laškarin, G., Faust, Z., Strbo, N., Sotošek, V., Szekeres-Bartho, J., Podack, E. R., & Rukavina, D. (1999). Progesterone directly and indirectly affects perforin expression in cytolytic cells. American Journal of Reproductive Immunology, 42(5), 312-320.

Progesterone directly and indirectly affects perforin expression in cytolytic cells. / Laškarin, G.; Faust, Zs; Strbo, Natasa; Sotošek, V.; Szekeres-Bartho, J.; Podack, E. R.; Rukavina, Daniel.

In: American Journal of Reproductive Immunology, Vol. 42, No. 5, 01.11.1999, p. 312-320.

Research output: Contribution to journalArticle

Laškarin, G, Faust, Z, Strbo, N, Sotošek, V, Szekeres-Bartho, J, Podack, ER & Rukavina, D 1999, 'Progesterone directly and indirectly affects perforin expression in cytolytic cells', American Journal of Reproductive Immunology, vol. 42, no. 5, pp. 312-320.
Laškarin G, Faust Z, Strbo N, Sotošek V, Szekeres-Bartho J, Podack ER et al. Progesterone directly and indirectly affects perforin expression in cytolytic cells. American Journal of Reproductive Immunology. 1999 Nov 1;42(5):312-320.
Laškarin, G. ; Faust, Zs ; Strbo, Natasa ; Sotošek, V. ; Szekeres-Bartho, J. ; Podack, E. R. ; Rukavina, Daniel. / Progesterone directly and indirectly affects perforin expression in cytolytic cells. In: American Journal of Reproductive Immunology. 1999 ; Vol. 42, No. 5. pp. 312-320.
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