Production of interleukin-1β by periodic acid-oxidized human peripheral blood mononuclear cells

George I. Malinin, Francis J. Hornicek, Theodore I. Malinin

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Interleukin-1 (IL-1) production by periodic acid (H5IO6)-oxidized human peripheral blood mononuclear (PBMN) cells was assessed by the thymocyte co-mitogenesis assay. Maximum IL-1 levels (∼ 1.2 U/ml) in the conditioned media of PBMN cells were registered within the first 24 hrs post-oxidation, whereas no IL-1 was detected in the media from 24 hrs control cultures. Thymocyte proliferation, driven by periodic acid-induced IL-1, was abolished by an antibody to IL-1alpha and IL-1β. Quantitative analysis of IL-1-containing medium by radioimmunoassay (RIA) indicated that IL-1β comprised about 80% of total IL-1. Partial characterization of H5IO6-induced IL-1β indicated that it was identical to IL-1 produced by lipopolysaccharide-stimulated macrophages. It is concluded that oxidation of human PBMN cells by H5IO6 triggers synthesis and release of IL-1, most of which was in its IL-1β form.

Original languageEnglish (US)
Pages (from-to)177-182
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume95
Issue number2
DOIs
StatePublished - May 1 1990

Keywords

  • cells
  • interleukin-1
  • membrane
  • oxidation
  • periodate

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

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