Production of cross-reactive peptide antibodies against viral capsid proteins of human enterovirus B to apply diagnostic reagent

Soo Young Jeong, Jeonghyun Ahn, Young Joo Cho, Yeun Jung Kim, Dae Sun Kim, Youngmee Jee, Heuiran Lee, Jae Hwan Nam

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The coxsackievirus group B (CVB) of the genus Enterovirus and the species human enterovirus B is a nonenveloped virus containing a single-stranded positive-sense RNA genome. Coxsackievirus has icosahedral symmetry and four capsid proteins, VP1, VP2, VP3, and VP4. Specific antibodies against each viral protein are prerequisites for various studies. In this study, we developed seven peptide-derived antibodies directed against coxsackievirus VP1 (NO1-NO5), VP2 (B3), and VP3 (GL3). We developed a type-specific antibody (NO1) and broadly cross-reactive antibodies (NO3 and NO5) to VP1. Anti-VP2 and anti-VP3 antibodies (B3 and GL3, respectively) are also cross-reactive to human enterovirus B such as CVB and echoviruses. Their sensitivities and reactivities are likely to be better than those of the commercial VP1 monoclonal antibody (MAb). The dot-blot analysis also showed that NO5 against VP1 is able to detect less than 1 μg [2×106 plaque-forming unit (pfu) of CVB3] of viruses, suggesting that it could be used to develop a diagnostic kit that can directly detect human enterovirus B. The antibodies produced here may allow us to undertake several studies, such as those involving viral trafficking, expression kinetics, and the roles of viral proteins in infection, and the development of diagnostic kits.

Original languageEnglish (US)
Pages (from-to)1091-1098
Number of pages8
JournalMicrobiology and Immunology
Volume51
Issue number11
DOIs
StatePublished - 2007

Keywords

  • Coxsackievirus
  • Dot blot
  • Human enterovirus B
  • Peptide antibody

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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