Production of arachidonic acid metabolites by endothelial cells in hyperoxia

Robert Jackson, D. B. Chandler, J. D. Fulmer

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

This study investigated the response of bovine pulmonary artery endothelial cells to incubation in hyperoxia (95% O2-5% CO2). Changes in cell number and morphology, release of lactate dehydrogenase, and production of arachidonic acid metabolites were assessed during continuous exposure of confluent endothelial monolayers to air (air-5% CO2, 'controls') or O2 (95% O2-5% CO2, 'O2-exposed') for periods of 12-72 h. Control monolayer cell numbers remained constant (~2,000,000 cells/flask), whereas the number of cells in O2-exposed monolayers decreased progressively to 30% of controls (P < 0.01) by 72 h. As assessed by radioimmunoassay, both control and O2-exposed cells produced the prostacyclin metabolite, 6-ketoprostaglandin F(1α) (6-keto-PGF(1α)), and prostaglandin F(2α) (PGF(2α)), but no thomboxane metabolite (TxB2) was detected. The O2-exposed cells released significantly more 6-keto-PGF(1α) and PGF(2α) than control cells when apparent net production rates over the entire 72-h period were compared. In addition, both control and O2-exposed (48 h) endothelial monolayers released immunoreactive leukotriene B4 (LTB4) on stimulation with calcium ionophore (10 μM A23187). As with the cyclooxygenase products, O2-exposed cells released more immunoreactive LTB4 than did controls. Both cyclooxygenase and lipoxygenase metabolites of arachidonic acid are released by cultured endothelial cells during the development of O2 toxicity.

Original languageEnglish
Pages (from-to)584-591
Number of pages8
JournalJournal of Applied Physiology
Volume61
Issue number2
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Hyperoxia
Prostaglandins F
Arachidonic Acid
Endothelial Cells
Leukotriene B4
Cell Count
Prostaglandin-Endoperoxide Synthases
Air
Arachidonate Lipoxygenases
Calcium Ionophores
Calcimycin
Epoprostenol
L-Lactate Dehydrogenase
Pulmonary Artery
Radioimmunoassay
Cultured Cells

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Production of arachidonic acid metabolites by endothelial cells in hyperoxia. / Jackson, Robert; Chandler, D. B.; Fulmer, J. D.

In: Journal of Applied Physiology, Vol. 61, No. 2, 01.01.1986, p. 584-591.

Research output: Contribution to journalArticle

Jackson, Robert ; Chandler, D. B. ; Fulmer, J. D. / Production of arachidonic acid metabolites by endothelial cells in hyperoxia. In: Journal of Applied Physiology. 1986 ; Vol. 61, No. 2. pp. 584-591.
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