Production and characterization of recombinant canine interferon-γ from Escherichia coli

K. Zucker, P. Lu, D. Asthana, M. Carreno, W. C. Yang, V. Esquenazi, L. Fuller, J. Miller

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We have used the recently cloned cDNA for canine interferon-γ (IFN-γ) to engineer bacteria to produce large amounts of the recombinant cytokine. The resulting protein can be recognized by monoclonal and polyclonal antibodies largely species specific for canine IFN-γ. The purified recombinant IFN-γ (rIFN-γ) also had biological activity in vitro in three assay systems: (i) vesicular stomatitis virus plaque inhibition, (ii) class II major histocompatibility complex antigen upregulation on canine kidney parenchymal cells, and (iii) amplification of in vitro tissue-associated lymphoproliferation, all known to be effected by native IFN-γ (nIFN-γ). The availability of large amounts of active canine rIFN-γ will be an important tool in studies of the role of this cytokine in the widely used experimental canine organ transplant model and also will be of diagnostic and therapeutic veterinary interest.

Original languageEnglish
Pages (from-to)91-97
Number of pages7
JournalJournal of Interferon Research
Volume13
Issue number2
StatePublished - Jan 1 1993
Externally publishedYes

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Interferons
Canidae
Escherichia coli
Cytokines
Vesicular Stomatitis
Histocompatibility Antigens Class II
Major Histocompatibility Complex
Up-Regulation
Complementary DNA
Monoclonal Antibodies
Viruses
Bacteria
Transplants
Kidney
Proteins
In Vitro Techniques
Therapeutics

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Zucker, K., Lu, P., Asthana, D., Carreno, M., Yang, W. C., Esquenazi, V., ... Miller, J. (1993). Production and characterization of recombinant canine interferon-γ from Escherichia coli. Journal of Interferon Research, 13(2), 91-97.

Production and characterization of recombinant canine interferon-γ from Escherichia coli. / Zucker, K.; Lu, P.; Asthana, D.; Carreno, M.; Yang, W. C.; Esquenazi, V.; Fuller, L.; Miller, J.

In: Journal of Interferon Research, Vol. 13, No. 2, 01.01.1993, p. 91-97.

Research output: Contribution to journalArticle

Zucker, K, Lu, P, Asthana, D, Carreno, M, Yang, WC, Esquenazi, V, Fuller, L & Miller, J 1993, 'Production and characterization of recombinant canine interferon-γ from Escherichia coli', Journal of Interferon Research, vol. 13, no. 2, pp. 91-97.
Zucker K, Lu P, Asthana D, Carreno M, Yang WC, Esquenazi V et al. Production and characterization of recombinant canine interferon-γ from Escherichia coli. Journal of Interferon Research. 1993 Jan 1;13(2):91-97.
Zucker, K. ; Lu, P. ; Asthana, D. ; Carreno, M. ; Yang, W. C. ; Esquenazi, V. ; Fuller, L. ; Miller, J. / Production and characterization of recombinant canine interferon-γ from Escherichia coli. In: Journal of Interferon Research. 1993 ; Vol. 13, No. 2. pp. 91-97.
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