Procurement of the human pancreas for pancreatic islet transplantation

Timothy C. Lee, Neal R. Barshes, F. Charles Brunicardi, Rodolfo Alejandro, Camillo Ricordi, Liz Nguyen, John A. Goss

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Background. The full potential of pancreatic islet transplantation (PIT) has not been realized because of the difficulties associated with islet isolation, particularly when associated with a remote islet isolation center. Herein, we describe the principles of pancreatic procurement for PIT, which have allowed us to achieve a successful pancreatic islet isolation rate 67% of the time when using a remote islet isolation center. Methods. Between January 16, 2002 and June 30, 2003, 39 pancreata were procured and processed for PIT at a distant islet isolation center. All pancreata were procured by a single surgeon, and special attention was given to careful dissection of the pancreas, maintenance of arterial inflow and the pressure differential between arterial and venous systems during perfusion, rapid organ cooling, and rapid removal of the pancreas from the body. Results. Twenty-six of 39 (67%) procured pancreata yielded more tha 5,000 islet equivalents (IEQ)/kg recipient weight and were transplanted. Median IEQs per isolation was 413,867, whereas median purity and viability were 65% and 100%, respectively. The median time for pancreatic excision was 34 minutes, whereas cold ischemia time was 6 hours and 40 minutes. Discussion. The principles we have adopted for pancreatic procurement for PIT have resulted in a 67% islet isolation success rate despite the maintenance of more than 5,000 IEQ/kg and the use of a remote islet isolation center.

Original languageEnglish
Pages (from-to)481-483
Number of pages3
JournalTransplantation
Volume78
Issue number3
DOIs
StatePublished - Aug 15 2004

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Islets of Langerhans Transplantation
Pancreas
Maintenance
Cold Ischemia
Islets of Langerhans
Dissection
Arterial Pressure
Perfusion
Weights and Measures

Keywords

  • Pancreas transplant
  • Pancreatic islet
  • Pancreatic procurement

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Procurement of the human pancreas for pancreatic islet transplantation. / Lee, Timothy C.; Barshes, Neal R.; Brunicardi, F. Charles; Alejandro, Rodolfo; Ricordi, Camillo; Nguyen, Liz; Goss, John A.

In: Transplantation, Vol. 78, No. 3, 15.08.2004, p. 481-483.

Research output: Contribution to journalArticle

Lee, Timothy C. ; Barshes, Neal R. ; Brunicardi, F. Charles ; Alejandro, Rodolfo ; Ricordi, Camillo ; Nguyen, Liz ; Goss, John A. / Procurement of the human pancreas for pancreatic islet transplantation. In: Transplantation. 2004 ; Vol. 78, No. 3. pp. 481-483.
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AB - Background. The full potential of pancreatic islet transplantation (PIT) has not been realized because of the difficulties associated with islet isolation, particularly when associated with a remote islet isolation center. Herein, we describe the principles of pancreatic procurement for PIT, which have allowed us to achieve a successful pancreatic islet isolation rate 67% of the time when using a remote islet isolation center. Methods. Between January 16, 2002 and June 30, 2003, 39 pancreata were procured and processed for PIT at a distant islet isolation center. All pancreata were procured by a single surgeon, and special attention was given to careful dissection of the pancreas, maintenance of arterial inflow and the pressure differential between arterial and venous systems during perfusion, rapid organ cooling, and rapid removal of the pancreas from the body. Results. Twenty-six of 39 (67%) procured pancreata yielded more tha 5,000 islet equivalents (IEQ)/kg recipient weight and were transplanted. Median IEQs per isolation was 413,867, whereas median purity and viability were 65% and 100%, respectively. The median time for pancreatic excision was 34 minutes, whereas cold ischemia time was 6 hours and 40 minutes. Discussion. The principles we have adopted for pancreatic procurement for PIT have resulted in a 67% islet isolation success rate despite the maintenance of more than 5,000 IEQ/kg and the use of a remote islet isolation center.

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