Procollagen alpha 1 type I

A potential aide in histopathological grading of glioma

Niramol Savaraj, Chunjing Wu, Howard Landy, Medhi Wangpaijit, Yingjie Wei, M. Tien Kuo, Carlos Robles, A. J. Furst, Theodore Lampidis, Lynn G Feun

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Collagen type I production has been shown to play a role in malignant transformation. We examined procollagen type I expression in brain tumors and with histopathological grading. Expression levels of procollagen alpha 1 type 1 were determined in 5 glioma cell lines by RT-PCR, Northern, and Western blot analysis. In addition, 41 primary brain tumors and 2 metastatic lung cancers to the brain were examined by PCR. Of the 5 glioma cell line analyzed, 3 (glioma 1, SW-1783 and U-118) expressed procollagen alpha 1 type I and were sensitive to vitamin D3 (VD3). In contrast, 2 of the cell lines (U-373 and T-98G) lacked procollagen alpha 1 type 1 expression. In patients' samples, 14 of 15 anaplastic and low grade gliomas expressed procollagen alpha 1 type I, and 12 of the 14 expressed high levels. In contrast, only 12 of 21 high grade gliomas from patients expressed procollagen alpha 1 type1 and among these, only 4 of the 12 expressed high levels. Thus, there is an inversed correlation between procollagen alpha 1 type 1 expression and histopathological grading (R 2 = -0.56, p = 0.0005). Our data suggest that procollagen alpha 1 type I expression occurs more commonly in intermediate and low grade gliomas and may assist in histopathological grading.

Original languageEnglish
Pages (from-to)577-581
Number of pages5
JournalCancer Investigation
Volume23
Issue number7
DOIs
StatePublished - Dec 1 2005

Fingerprint

Collagen Type I
Glioma
Brain Neoplasms
Cell Line
Procollagen
Polymerase Chain Reaction
Cholecalciferol
Northern Blotting
Lung Neoplasms
Western Blotting

Keywords

  • Glioma
  • Histopathological grading
  • Procollagen

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Procollagen alpha 1 type I : A potential aide in histopathological grading of glioma. / Savaraj, Niramol; Wu, Chunjing; Landy, Howard; Wangpaijit, Medhi; Wei, Yingjie; Kuo, M. Tien; Robles, Carlos; Furst, A. J.; Lampidis, Theodore; Feun, Lynn G.

In: Cancer Investigation, Vol. 23, No. 7, 01.12.2005, p. 577-581.

Research output: Contribution to journalArticle

Savaraj, Niramol ; Wu, Chunjing ; Landy, Howard ; Wangpaijit, Medhi ; Wei, Yingjie ; Kuo, M. Tien ; Robles, Carlos ; Furst, A. J. ; Lampidis, Theodore ; Feun, Lynn G. / Procollagen alpha 1 type I : A potential aide in histopathological grading of glioma. In: Cancer Investigation. 2005 ; Vol. 23, No. 7. pp. 577-581.
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