Procollagen alpha 1 type I: A potential aide in histopathological grading of glioma

Niramol Savaraj, Chunjing Wu, Howard Landy, Medhi Wangpaijit, Yingjie Wei, M. Tien Kuo, Carlos Robles, A. J. Furst, Theodore Lampidis, Lynn Feun

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Collagen type I production has been shown to play a role in malignant transformation. We examined procollagen type I expression in brain tumors and with histopathological grading. Expression levels of procollagen alpha 1 type 1 were determined in 5 glioma cell lines by RT-PCR, Northern, and Western blot analysis. In addition, 41 primary brain tumors and 2 metastatic lung cancers to the brain were examined by PCR. Of the 5 glioma cell line analyzed, 3 (glioma 1, SW-1783 and U-118) expressed procollagen alpha 1 type I and were sensitive to vitamin D3 (VD3). In contrast, 2 of the cell lines (U-373 and T-98G) lacked procollagen alpha 1 type 1 expression. In patients' samples, 14 of 15 anaplastic and low grade gliomas expressed procollagen alpha 1 type I, and 12 of the 14 expressed high levels. In contrast, only 12 of 21 high grade gliomas from patients expressed procollagen alpha 1 type1 and among these, only 4 of the 12 expressed high levels. Thus, there is an inversed correlation between procollagen alpha 1 type 1 expression and histopathological grading (R 2 = -0.56, p = 0.0005). Our data suggest that procollagen alpha 1 type I expression occurs more commonly in intermediate and low grade gliomas and may assist in histopathological grading.

Original languageEnglish (US)
Pages (from-to)577-581
Number of pages5
JournalCancer Investigation
Volume23
Issue number7
DOIs
StatePublished - Dec 1 2005

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Keywords

  • Glioma
  • Histopathological grading
  • Procollagen

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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