Probing skin pigmentation changes with transient absorption imaging of eumelanin and pheomelanin

Dan Fu, Tong Ye, Thomas E. Matthews, James M Grichnik, Lian Hong, John D. Simon, Warren S. Warren

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

As some of the most ubiquitous and biologically important natural pigments, melanins play essential roles in the photoprotection of skin. Changes in melanin production could potentially be useful for clinical diagnosis of the progression stage of melanoma. Previously we demonstrated a new method for imaging melanin distribution in tissue with two-color transient absorption microscopy. Here we extend this study to longer wavelengths and show that we are able to image melanin in fixed thin skin slices with higher signal-to-noise ratios (SNRs) and demonstrate epimode imaging. We show that both photothermal effects and long-lived excited states can contribute to the long-lived signal. Eumelanin and pheomelanin exhibit markedly different long-lived excited state absorption. This difference should enable us to map out their respective distribution in tissue samples with subcellular resolution. This technique could provide valuable information in diagnosing the malignant transformation of melanocytes.

Original languageEnglish
Article number054036
JournalJournal of Biomedical Optics
Volume13
Issue number5
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

Fingerprint

Melanin
melanin
Melanins
Skin
Imaging techniques
Excited states
Tissue
pigments
Pigments
progressions
excitation
Signal to noise ratio
Microscopic examination
signal to noise ratios
microscopy
Color
color
Wavelength
pheomelanin
eumelanin

Keywords

  • excited state absorption
  • melanoma imaging
  • multiphoton microscopy
  • photothermal effects
  • transient absorption
  • two-photon absorption

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Fu, D., Ye, T., Matthews, T. E., Grichnik, J. M., Hong, L., Simon, J. D., & Warren, W. S. (2008). Probing skin pigmentation changes with transient absorption imaging of eumelanin and pheomelanin. Journal of Biomedical Optics, 13(5), [054036]. https://doi.org/10.1117/1.2976424

Probing skin pigmentation changes with transient absorption imaging of eumelanin and pheomelanin. / Fu, Dan; Ye, Tong; Matthews, Thomas E.; Grichnik, James M; Hong, Lian; Simon, John D.; Warren, Warren S.

In: Journal of Biomedical Optics, Vol. 13, No. 5, 054036, 01.12.2008.

Research output: Contribution to journalArticle

Fu, D, Ye, T, Matthews, TE, Grichnik, JM, Hong, L, Simon, JD & Warren, WS 2008, 'Probing skin pigmentation changes with transient absorption imaging of eumelanin and pheomelanin', Journal of Biomedical Optics, vol. 13, no. 5, 054036. https://doi.org/10.1117/1.2976424
Fu, Dan ; Ye, Tong ; Matthews, Thomas E. ; Grichnik, James M ; Hong, Lian ; Simon, John D. ; Warren, Warren S. / Probing skin pigmentation changes with transient absorption imaging of eumelanin and pheomelanin. In: Journal of Biomedical Optics. 2008 ; Vol. 13, No. 5.
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