Probenecid, a gout remedy, inhibits pannexin 1 channels

William Silverman, Silviu Locovei, Gerhard Dahl

Research output: Contribution to journalArticle

244 Scopus citations

Abstract

Probenecid is a well-established drug for the treatment of gout and is thought to act on an organic anion transporter, thereby affecting uric acid excretion in the kidney by blocking urate reuptake. Probenecid also has been shown to affect ATP release, leading to the suggestion that ATP release involves an organic anion transporter. Other pharmacological evidence and the observation of dye uptake, however, suggest that the nonvesicular release of ATP is mediated by large membrane channels, with pannexin 1 being a prominent candidate. In the present study we show that probenecid inhibited currents mediated by pannexin 1 channels in the same concentration range as observed for inhibition of transport processes. Probenecid did not affect channels formed by connexins. Thus probenecid allows for discrimination between channels formed by connexins and pannexins.

Original languageEnglish (US)
Pages (from-to)C761-C767
JournalAmerican Journal of Physiology - Cell Physiology
Volume295
Issue number3
DOIs
StatePublished - Sep 1 2008

    Fingerprint

Keywords

  • ATP release
  • Connexin
  • Erythrocyte
  • Transport

ASJC Scopus subject areas

  • Cell Biology
  • Physiology

Cite this