PRL gene expression in individual living mammotropes displays distinct functional pulses that oscillate in a noncircadian temporal pattern

Spencer L. Shorte, Gilles M. Leclerc, Rafael Vazquez-Martinez, David C. Leaumont, William J. Faught, L. Stephen Frawley, Fredric R. Boockfor

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

PRL gene expression in the anterior pituitary has been the focus of intensive investigation for many years, but very little information is available on the actual dynamics by which this process occurs in individual mammotrope cells. Here, we used single cell bioluminescent imaging microscopy and a recently refined reporter gene strategy to measure PRL promoter-driven gene expression (PRL-GE) in individual living primary mammotropes. Using this approach we report a new phenomenon involving repetitive on/off gene expression bursts that occurred in a distinctly noncircadian oscillatory pattern. Furthermore, we demonstrate a functional basis for these gene expression oscillations, inasmuch as PRL-GE pulses were sensitive to calcium-dependent modulation, which we show arose exclusively as changes in the shape of individual pulse episodes. Our results provide the first clear evidence that PRL-GE, in its homologous cell environment, displays oscillatory bursts of activity. Moreover, they strongly support the idea that these discrete on/off bursts of activity serve as an important determinant of the timing and level of PRL-GE under both basal and stimulated conditions.

Original languageEnglish
Pages (from-to)1126-1133
Number of pages8
JournalEndocrinology
Volume143
Issue number3
DOIs
StatePublished - Mar 4 2002
Externally publishedYes

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Gene Expression
Reporter Genes
Microscopy
Calcium

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Shorte, S. L., Leclerc, G. M., Vazquez-Martinez, R., Leaumont, D. C., Faught, W. J., Stephen Frawley, L., & Boockfor, F. R. (2002). PRL gene expression in individual living mammotropes displays distinct functional pulses that oscillate in a noncircadian temporal pattern. Endocrinology, 143(3), 1126-1133. https://doi.org/10.1210/en.143.3.1126

PRL gene expression in individual living mammotropes displays distinct functional pulses that oscillate in a noncircadian temporal pattern. / Shorte, Spencer L.; Leclerc, Gilles M.; Vazquez-Martinez, Rafael; Leaumont, David C.; Faught, William J.; Stephen Frawley, L.; Boockfor, Fredric R.

In: Endocrinology, Vol. 143, No. 3, 04.03.2002, p. 1126-1133.

Research output: Contribution to journalArticle

Shorte, SL, Leclerc, GM, Vazquez-Martinez, R, Leaumont, DC, Faught, WJ, Stephen Frawley, L & Boockfor, FR 2002, 'PRL gene expression in individual living mammotropes displays distinct functional pulses that oscillate in a noncircadian temporal pattern', Endocrinology, vol. 143, no. 3, pp. 1126-1133. https://doi.org/10.1210/en.143.3.1126
Shorte, Spencer L. ; Leclerc, Gilles M. ; Vazquez-Martinez, Rafael ; Leaumont, David C. ; Faught, William J. ; Stephen Frawley, L. ; Boockfor, Fredric R. / PRL gene expression in individual living mammotropes displays distinct functional pulses that oscillate in a noncircadian temporal pattern. In: Endocrinology. 2002 ; Vol. 143, No. 3. pp. 1126-1133.
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