Primordial germ cell isolation from xenopus laevis embryos

Amanda M. Butler, Tristan Aguero, Karen M. Newman, Mary Lou L King

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Primordial germ cells (PGCs) are the precursors to the gametes and have the unique ability to retain full developmental potential. However, the mechanism(s) and gene-network(s) necessary for their proper specification and development are poorly understood. This is due, in part, to the challenges that must be overcome in order to identify and isolate PGCs during critical stages of development. Two distinct mechanisms have been characterized to specify the germ cell lineage in vertebrates: induction and inheritance. Regardless of mechanism, there are common developmental features shared among all vertebrates in forming the germ cell lineage. Xenopus offers several advantages for understanding the molecular mechanisms necessary to establish the germ line. Here, we provide detailed methods for isolating live PGCs at different time points: 1) just after they have segregated from the endodermal lineage, and 2) while they are migrating towards the presumptive gonad. Isolation of PGCs at these critical developmental stages will allow for the investigation of the mechanism(s) and gene-network(s) necessary for their proper specification and development.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages115-124
Number of pages10
Volume1463
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1463
ISSN (Print)10643745

Fingerprint

Cell Separation
Xenopus laevis
Germ Cells
Embryonic Structures
Gene Regulatory Networks
Cell Lineage
Vertebrates
Gonads
Xenopus

Keywords

  • Germ line
  • Germ plasm
  • Maternal-to-zygotic transition (MZT)
  • Migration
  • Primordial germ cell (PGC)
  • Xenopus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Butler, A. M., Aguero, T., Newman, K. M., & King, M. L. L. (2017). Primordial germ cell isolation from xenopus laevis embryos. In Methods in Molecular Biology (Vol. 1463, pp. 115-124). (Methods in Molecular Biology; Vol. 1463). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-4017-2_9

Primordial germ cell isolation from xenopus laevis embryos. / Butler, Amanda M.; Aguero, Tristan; Newman, Karen M.; King, Mary Lou L.

Methods in Molecular Biology. Vol. 1463 Humana Press Inc., 2017. p. 115-124 (Methods in Molecular Biology; Vol. 1463).

Research output: Chapter in Book/Report/Conference proceedingChapter

Butler, AM, Aguero, T, Newman, KM & King, MLL 2017, Primordial germ cell isolation from xenopus laevis embryos. in Methods in Molecular Biology. vol. 1463, Methods in Molecular Biology, vol. 1463, Humana Press Inc., pp. 115-124. https://doi.org/10.1007/978-1-4939-4017-2_9
Butler AM, Aguero T, Newman KM, King MLL. Primordial germ cell isolation from xenopus laevis embryos. In Methods in Molecular Biology. Vol. 1463. Humana Press Inc. 2017. p. 115-124. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-4017-2_9
Butler, Amanda M. ; Aguero, Tristan ; Newman, Karen M. ; King, Mary Lou L. / Primordial germ cell isolation from xenopus laevis embryos. Methods in Molecular Biology. Vol. 1463 Humana Press Inc., 2017. pp. 115-124 (Methods in Molecular Biology).
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