Abstract
After PCR amplification two overlapping cDNA clones encoding the dog homologue of the human CD4 glycoprotein were identified. This internal fragment of the mature protein including the complete extracellular domains, consists of 1297 bp with a deduced amino acid sequence of 432 residues. The dog CD4 molecule differs from the corresponding protein of other species including human in the second domain. We found nine extra residues in the beginning of that domain, a cysteine at position 138, usually involved in a disulfide bridge, is substituted by tryptophan, and three new glycosylation sites are present.
| Original language | English |
|---|---|
| Pages (from-to) | 315-318 |
| Number of pages | 4 |
| Journal | BBA - Gene Structure and Expression |
| Volume | 1172 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 20 1993 |
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Keywords
- (Dog thymus)
- cDNA cloning
- Ig superfamily
- Polymerase chain reaction
- RNA
- Sequence homology
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Genetics
- Structural Biology
Cite this
Primary structure of the canine CD4 antigen. / Milde, Kerstin F.; Conner, Gregory E; Mintz, Daniel H.; Alejandro, Rodolfo.
In: BBA - Gene Structure and Expression, Vol. 1172, No. 3, 20.03.1993, p. 315-318.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Primary structure of the canine CD4 antigen
AU - Milde, Kerstin F.
AU - Conner, Gregory E
AU - Mintz, Daniel H.
AU - Alejandro, Rodolfo
PY - 1993/3/20
Y1 - 1993/3/20
N2 - After PCR amplification two overlapping cDNA clones encoding the dog homologue of the human CD4 glycoprotein were identified. This internal fragment of the mature protein including the complete extracellular domains, consists of 1297 bp with a deduced amino acid sequence of 432 residues. The dog CD4 molecule differs from the corresponding protein of other species including human in the second domain. We found nine extra residues in the beginning of that domain, a cysteine at position 138, usually involved in a disulfide bridge, is substituted by tryptophan, and three new glycosylation sites are present.
AB - After PCR amplification two overlapping cDNA clones encoding the dog homologue of the human CD4 glycoprotein were identified. This internal fragment of the mature protein including the complete extracellular domains, consists of 1297 bp with a deduced amino acid sequence of 432 residues. The dog CD4 molecule differs from the corresponding protein of other species including human in the second domain. We found nine extra residues in the beginning of that domain, a cysteine at position 138, usually involved in a disulfide bridge, is substituted by tryptophan, and three new glycosylation sites are present.
KW - (Dog thymus)
KW - cDNA cloning
KW - Ig superfamily
KW - Polymerase chain reaction
KW - RNA
KW - Sequence homology
UR - http://www.scopus.com/inward/record.url?scp=0027537366&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027537366&partnerID=8YFLogxK
U2 - 10.1016/0167-4781(93)90220-8
DO - 10.1016/0167-4781(93)90220-8
M3 - Article
VL - 1172
SP - 315
EP - 318
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 3
ER -