Primary neurons become less susceptible to coxsackievirus B5 following maturation: The correlation with the decreased level of CAR expression on cell surface

Jeonghyun Ahn, Youngmee Jee, Ilseon Seo, Yong Yoon Seung, Dong Hou Kim, Kyum Kim Yoo, Heuiran Lee

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Coxsackievirus B (CVB) is one of the major pathogens of aseptic meningitis and meningioencephalitis, particularly in newborn infants. To analyze the influence of neural maturation on susceptibility to CVB infection, we prepared immature and mature neurons from 16-day-old BALB/c embryonic cortex. In contrast to immature neurons, mature neurons were less susceptible to CVB5 infection, as indicated by the decrease of cytopathic features. In mature neurons, progeny virus production was significantly hindered, and virus capsid protein VP1 synthesis and virus genome amplification were concomitantly reduced. In addition, the expression of coxsackievirus and adenovirus receptor (CAR), the major receptor of CVB5, was down-regulated in mature neurons. The antibody treatment specific to CAR significantly attenuated CVB5 susceptibility of immature neurons. These findings demonstrate that mature neurons become less susceptible to CVB by the decrease of CAR level. Thus, the data strongly support the idea thatthe level of virus receptor in neurons is one of the crucial determinants in the age-dependency of CVB virulence in central nervous system.

Original languageEnglish (US)
Pages (from-to)434-440
Number of pages7
JournalJournal of Medical Virology
Volume80
Issue number3
DOIs
StatePublished - Mar 2008

Keywords

  • Coxsackievirus B
  • Cytotoxicity
  • Primary neurons
  • Receptor
  • Virus replication

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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