Abstract
OBJECTIVE: Our purpose was to test the ability of an antiserum to vascular permeability factor/vascular endothelial growth factor to inhibit postoperative adhesion formation in a murine model. STUDY DESIGN: After a standardized peritoneal injury, 28 Balb/c mice were randomized and treated intraperitoneally with either vascular permeability factor antiserum (n = 14) or preimmune serum (n = 14) at the time of abdominal closure. Mice were killed on postoperative day 14, and the development of intraabdominal adhesions was determined. Adhesion scoring was based on an overall assessment of the extent, location, and type of adhesions present. Statistical analyses were performed with the Mann-Whitney and Fisher's exact tests. RESULTS: The mice treated with the vascular permeability factor antiserum had significantly lower adhesion scores than did the control group (mean ± SD 1.5 ± 0.9, median 1.0, vs control 2.5 ± 0.7, median 3.0). When the groups were analyzed for the presence of grade 2 or 3 adhesions, the group treated with vascular permeability factor antiserum had a significantly lower incidence of advanced adhesions (38%, vs control 92%). CONCLUSION: This study demonstrates that the intraperitoneal administration of a neutralizing antiserum to vascular permeability factor/vascular endothelial growth factor limits postoperative adhesion formation. These observations, to the best of our knowledge, are the first to suggest a role for vascular permeability factor in the pathogenesis of adhesion formation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1502-1506 |
| Number of pages | 5 |
| Journal | American Journal of Obstetrics and Gynecology |
| Volume | 174 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 1996 |
| Externally published | Yes |
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Keywords
- adhesions
- Balb/c mice
- vascular endothelial growth factor
- vascular hypermeability
- Vascular permeability factor
ASJC Scopus subject areas
- Obstetrics and Gynecology
Cite this
Prevention of postoperative adhesions by an antibody to vascular permeability factor/vascular endothelial growth factor in a murine model. / Saltzman, A. K.; Olson, T. A.; Mohanraj, D.; Carson, L. F.; Ramakrishnan, Sundaram.
In: American Journal of Obstetrics and Gynecology, Vol. 174, No. 5, 01.01.1996, p. 1502-1506.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prevention of postoperative adhesions by an antibody to vascular permeability factor/vascular endothelial growth factor in a murine model
AU - Saltzman, A. K.
AU - Olson, T. A.
AU - Mohanraj, D.
AU - Carson, L. F.
AU - Ramakrishnan, Sundaram
PY - 1996/1/1
Y1 - 1996/1/1
N2 - OBJECTIVE: Our purpose was to test the ability of an antiserum to vascular permeability factor/vascular endothelial growth factor to inhibit postoperative adhesion formation in a murine model. STUDY DESIGN: After a standardized peritoneal injury, 28 Balb/c mice were randomized and treated intraperitoneally with either vascular permeability factor antiserum (n = 14) or preimmune serum (n = 14) at the time of abdominal closure. Mice were killed on postoperative day 14, and the development of intraabdominal adhesions was determined. Adhesion scoring was based on an overall assessment of the extent, location, and type of adhesions present. Statistical analyses were performed with the Mann-Whitney and Fisher's exact tests. RESULTS: The mice treated with the vascular permeability factor antiserum had significantly lower adhesion scores than did the control group (mean ± SD 1.5 ± 0.9, median 1.0, vs control 2.5 ± 0.7, median 3.0). When the groups were analyzed for the presence of grade 2 or 3 adhesions, the group treated with vascular permeability factor antiserum had a significantly lower incidence of advanced adhesions (38%, vs control 92%). CONCLUSION: This study demonstrates that the intraperitoneal administration of a neutralizing antiserum to vascular permeability factor/vascular endothelial growth factor limits postoperative adhesion formation. These observations, to the best of our knowledge, are the first to suggest a role for vascular permeability factor in the pathogenesis of adhesion formation.
AB - OBJECTIVE: Our purpose was to test the ability of an antiserum to vascular permeability factor/vascular endothelial growth factor to inhibit postoperative adhesion formation in a murine model. STUDY DESIGN: After a standardized peritoneal injury, 28 Balb/c mice were randomized and treated intraperitoneally with either vascular permeability factor antiserum (n = 14) or preimmune serum (n = 14) at the time of abdominal closure. Mice were killed on postoperative day 14, and the development of intraabdominal adhesions was determined. Adhesion scoring was based on an overall assessment of the extent, location, and type of adhesions present. Statistical analyses were performed with the Mann-Whitney and Fisher's exact tests. RESULTS: The mice treated with the vascular permeability factor antiserum had significantly lower adhesion scores than did the control group (mean ± SD 1.5 ± 0.9, median 1.0, vs control 2.5 ± 0.7, median 3.0). When the groups were analyzed for the presence of grade 2 or 3 adhesions, the group treated with vascular permeability factor antiserum had a significantly lower incidence of advanced adhesions (38%, vs control 92%). CONCLUSION: This study demonstrates that the intraperitoneal administration of a neutralizing antiserum to vascular permeability factor/vascular endothelial growth factor limits postoperative adhesion formation. These observations, to the best of our knowledge, are the first to suggest a role for vascular permeability factor in the pathogenesis of adhesion formation.
KW - adhesions
KW - Balb/c mice
KW - vascular endothelial growth factor
KW - vascular hypermeability
KW - Vascular permeability factor
UR - http://www.scopus.com/inward/record.url?scp=0029930838&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029930838&partnerID=8YFLogxK
U2 - 10.1016/S0002-9378(96)70596-3
DO - 10.1016/S0002-9378(96)70596-3
M3 - Article
C2 - 9065119
AN - SCOPUS:0029930838
VL - 174
SP - 1502
EP - 1506
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
SN - 0002-9378
IS - 5
ER -