Purpose. Recovery of Haemophilus influenzae from intraocular fluids, increasing frequency and spectrum of disease plus the emergence of multidrug resistant isolates is a cause for concern. Methods. A laboratory based surveillance was initiated for detection of invasive serotypes(grp B), shifting biotypes and drug resistance for H. influenzae recovered from ocular specimens. Isolates (299) recovered from patients seen at BPEI (Jan. 1990-Jun. 1995) were biotyped using the RapNH system. Disk diffusion and the "E" test were employed to determine and compare prevalence of drug resistance for Ampicillin and other antimicrobials. Results. Seventy percent of the isolates were from acute blepharoconjunctivitis, keratitis (8%) and intraocular fluids (5%). Lacrimal apparatus and soft tissues constituted 10%. Invasive serotypes were found in less than 1% of all patients. Ninety eight percent of the isolates were non-typable; predominantly biotypes I and IV. Haemophilus aegypticus and biotype III were recovered in only 6% of patients. 27% of total isolates were resistant to Ampicillin. Multidrug resistance was evident for 5% of isolates. Acute, moderately resistant isolates were most often recovered from children under the age of 5. Adult infections were characterized by chronicity and multidrug resistance. Conclusions. Haemophilus influenzae remains a serious ocular pathogen with expanded spectrum of disease and emerging multidrug resistance.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - Feb 15 1996|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience