TY - JOUR
T1 - Presynaptic neuronal antigens expressed by a small cell lung carcinoma cell line
AU - Benatar, Michael
AU - Blaes, Franz
AU - Johnston, Irene
AU - Wilson, Katherine
AU - Vincent, Angela
AU - Beeson, David
AU - Lang, Bethan
N1 - Funding Information:
M.B. was a Rhodes Scholar. F.B. was a recipient of a post-doctoral fellowship from the “Deutsche Forschunggsgemeinschaft” (Bl 452/1-1). The authors are very grateful to Professor John Newsom-Davis for providing the patients’ clinical information and to Ms. Eve Goodyer for the serum samples.
Funding Information:
This work was supported by grants from the MRC, Muscular Dystrophy Association, Jules Thorn Trust and the Myasthenia Gravis Association.
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2001/2/1
Y1 - 2001/2/1
N2 - Small cell lung carcinoma (SCLC) is a tumour of neuroendocrine origin often found in association with autoimmune paraneoplastic neurological disorders. We established a SCLC cell line from a woman with Lambert-Eaton myasthenic syndrome (LEMS) who developed antibodies to both the P/Q-type voltage-gated calcium channels (VGCC) and the muscle acetycholine receptor (AChR). We used a range of techniques to establish which neuronal antigens were expressed in her tumour cell line. The results show that many proteins involved in exocytosis are present in the SCLC cells, and that depolarisation-dependent release of [3H]-serotonin is linked to calcium influx through P/Q-type VGCCs. In addition, some of the subunits encoding the AChR and both agrin and ARIA, molecules released from the motor nerve during development, were expressed. These results suggest that many potential antigenic targets are present in SCLC, and indicate a surprising 'motor nerve terminal'-like characteristic of this line.
AB - Small cell lung carcinoma (SCLC) is a tumour of neuroendocrine origin often found in association with autoimmune paraneoplastic neurological disorders. We established a SCLC cell line from a woman with Lambert-Eaton myasthenic syndrome (LEMS) who developed antibodies to both the P/Q-type voltage-gated calcium channels (VGCC) and the muscle acetycholine receptor (AChR). We used a range of techniques to establish which neuronal antigens were expressed in her tumour cell line. The results show that many proteins involved in exocytosis are present in the SCLC cells, and that depolarisation-dependent release of [3H]-serotonin is linked to calcium influx through P/Q-type VGCCs. In addition, some of the subunits encoding the AChR and both agrin and ARIA, molecules released from the motor nerve during development, were expressed. These results suggest that many potential antigenic targets are present in SCLC, and indicate a surprising 'motor nerve terminal'-like characteristic of this line.
KW - Autoantibodies
KW - Paraneoplastic neurological disorders
KW - Small cell lung carcinoma
KW - Voltage-gated calcium channels
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U2 - 10.1016/S0165-5728(00)00431-8
DO - 10.1016/S0165-5728(00)00431-8
M3 - Article
C2 - 11137587
AN - SCOPUS:0035255064
VL - 113
SP - 153
EP - 162
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
SN - 0165-5728
IS - 1
ER -