Abstract
Small cell lung carcinoma (SCLC) is a tumour of neuroendocrine origin often found in association with autoimmune paraneoplastic neurological disorders. We established a SCLC cell line from a woman with Lambert-Eaton myasthenic syndrome (LEMS) who developed antibodies to both the P/Q-type voltage-gated calcium channels (VGCC) and the muscle acetycholine receptor (AChR). We used a range of techniques to establish which neuronal antigens were expressed in her tumour cell line. The results show that many proteins involved in exocytosis are present in the SCLC cells, and that depolarisation-dependent release of [3H]-serotonin is linked to calcium influx through P/Q-type VGCCs. In addition, some of the subunits encoding the AChR and both agrin and ARIA, molecules released from the motor nerve during development, were expressed. These results suggest that many potential antigenic targets are present in SCLC, and indicate a surprising 'motor nerve terminal'-like characteristic of this line.
| Original language | English |
|---|---|
| Pages (from-to) | 153-162 |
| Number of pages | 10 |
| Journal | Journal of Neuroimmunology |
| Volume | 113 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 2001 |
| Externally published | Yes |
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Keywords
- Autoantibodies
- Paraneoplastic neurological disorders
- Small cell lung carcinoma
- Voltage-gated calcium channels
ASJC Scopus subject areas
- Immunology
- Clinical Neurology
- Immunology and Allergy
- Neurology
Cite this
Presynaptic neuronal antigens expressed by a small cell lung carcinoma cell line. / Benatar, Michael G; Blaes, Franz; Johnston, Irene; Wilson, Katherine; Vincent, Angela; Beeson, David; Lang, Bethan.
In: Journal of Neuroimmunology, Vol. 113, No. 1, 01.02.2001, p. 153-162.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Presynaptic neuronal antigens expressed by a small cell lung carcinoma cell line
AU - Benatar, Michael G
AU - Blaes, Franz
AU - Johnston, Irene
AU - Wilson, Katherine
AU - Vincent, Angela
AU - Beeson, David
AU - Lang, Bethan
PY - 2001/2/1
Y1 - 2001/2/1
N2 - Small cell lung carcinoma (SCLC) is a tumour of neuroendocrine origin often found in association with autoimmune paraneoplastic neurological disorders. We established a SCLC cell line from a woman with Lambert-Eaton myasthenic syndrome (LEMS) who developed antibodies to both the P/Q-type voltage-gated calcium channels (VGCC) and the muscle acetycholine receptor (AChR). We used a range of techniques to establish which neuronal antigens were expressed in her tumour cell line. The results show that many proteins involved in exocytosis are present in the SCLC cells, and that depolarisation-dependent release of [3H]-serotonin is linked to calcium influx through P/Q-type VGCCs. In addition, some of the subunits encoding the AChR and both agrin and ARIA, molecules released from the motor nerve during development, were expressed. These results suggest that many potential antigenic targets are present in SCLC, and indicate a surprising 'motor nerve terminal'-like characteristic of this line.
AB - Small cell lung carcinoma (SCLC) is a tumour of neuroendocrine origin often found in association with autoimmune paraneoplastic neurological disorders. We established a SCLC cell line from a woman with Lambert-Eaton myasthenic syndrome (LEMS) who developed antibodies to both the P/Q-type voltage-gated calcium channels (VGCC) and the muscle acetycholine receptor (AChR). We used a range of techniques to establish which neuronal antigens were expressed in her tumour cell line. The results show that many proteins involved in exocytosis are present in the SCLC cells, and that depolarisation-dependent release of [3H]-serotonin is linked to calcium influx through P/Q-type VGCCs. In addition, some of the subunits encoding the AChR and both agrin and ARIA, molecules released from the motor nerve during development, were expressed. These results suggest that many potential antigenic targets are present in SCLC, and indicate a surprising 'motor nerve terminal'-like characteristic of this line.
KW - Autoantibodies
KW - Paraneoplastic neurological disorders
KW - Small cell lung carcinoma
KW - Voltage-gated calcium channels
UR - http://www.scopus.com/inward/record.url?scp=0035255064&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035255064&partnerID=8YFLogxK
U2 - 10.1016/S0165-5728(00)00431-8
DO - 10.1016/S0165-5728(00)00431-8
M3 - Article
C2 - 11137587
AN - SCOPUS:0035255064
VL - 113
SP - 153
EP - 162
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1
ER -