Presynaptic cytomatrix protein bassoon is localized at both excitatory and inhibitory synapses of rat brain

Karin Richter, Kristina Langnaese, Michael R. Kreutz, Gisela Olias, Rong G Zhai, Henning Scheich, Craig C. Garner, Eckart D. Gundelfinger

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Bassoon is a 420-kDa protein specifically localized at the active zone of presynaptic nerve terminals. It is thought to be involved in the structural organization of the neurotransmitter release site. We studied the distribution of Bassoon transcripts and protein in rat brain and assessed which types of presynaptic terminals contain the protein. As shown by in situ hybridization, Bassoon transcripts are widely distributed in the brain and occur primarily in excitatory neurons. In addition, examples of γ- aminobutyric acid (GABA)-ergic neurons expressing Bassoon are detected. At the light microscopic level, Bassoon immunoreactivity is found in synaptic neuropil regions throughout the brain, with the strongest expression in the hippocampus, the cerebellar cortex, and the olfactory bulb. Immunoelectron microscopy showed that Bassoon immunoreactivity is found in both asymmetric type 1 and symmetric type 2 synapses. Immunopositive asymmetric synapses include mossy fiber boutons and various spine and shaft synapses in the hippocampus and mossy fiber terminals and parallel fiber terminals in the cerebellum. Bassoon-containing symmetric synapses are observed, e.g., between basket and granule cells in the hippocampus, between Golgi cells and granule cells, and between basket cells and Purkinje cells in the cerebellum. Within synaptic terminals, Bassoon appears highly concentrated at sites opposite to postsynaptic densities. In cultured hippocampal neurons, Bassoon was found to colocalize with GABA(A) and glutamate (GluR1) receptors. These data indicate that Bassoon is a component of the presynaptic apparatus of both excitatory glutamatergic and inhibitory GABAergic synapses.

Original languageEnglish
Pages (from-to)437-448
Number of pages12
JournalJournal of Comparative Neurology
Volume408
Issue number3
DOIs
StatePublished - Jun 7 1999
Externally publishedYes

Fingerprint

Synapses
Presynaptic Terminals
Brain
Hippocampus
Proteins
Cerebellum
Aminobutyrates
Post-Synaptic Density
Neurons
GABAergic Neurons
Cerebellar Cortex
Neuropil
Immunoelectron Microscopy
Olfactory Bulb
Purkinje Cells
Glutamate Receptors
gamma-Aminobutyric Acid
In Situ Hybridization
Neurotransmitter Agents
Spine

Keywords

  • Cerebellum
  • GABA
  • Glutamate
  • Hippocampus
  • Neurotransmitters
  • Presynaptic terminal

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Presynaptic cytomatrix protein bassoon is localized at both excitatory and inhibitory synapses of rat brain. / Richter, Karin; Langnaese, Kristina; Kreutz, Michael R.; Olias, Gisela; Zhai, Rong G; Scheich, Henning; Garner, Craig C.; Gundelfinger, Eckart D.

In: Journal of Comparative Neurology, Vol. 408, No. 3, 07.06.1999, p. 437-448.

Research output: Contribution to journalArticle

Richter, Karin ; Langnaese, Kristina ; Kreutz, Michael R. ; Olias, Gisela ; Zhai, Rong G ; Scheich, Henning ; Garner, Craig C. ; Gundelfinger, Eckart D. / Presynaptic cytomatrix protein bassoon is localized at both excitatory and inhibitory synapses of rat brain. In: Journal of Comparative Neurology. 1999 ; Vol. 408, No. 3. pp. 437-448.
@article{085a632da3954fae944a491a19cae847,
title = "Presynaptic cytomatrix protein bassoon is localized at both excitatory and inhibitory synapses of rat brain",
abstract = "Bassoon is a 420-kDa protein specifically localized at the active zone of presynaptic nerve terminals. It is thought to be involved in the structural organization of the neurotransmitter release site. We studied the distribution of Bassoon transcripts and protein in rat brain and assessed which types of presynaptic terminals contain the protein. As shown by in situ hybridization, Bassoon transcripts are widely distributed in the brain and occur primarily in excitatory neurons. In addition, examples of γ- aminobutyric acid (GABA)-ergic neurons expressing Bassoon are detected. At the light microscopic level, Bassoon immunoreactivity is found in synaptic neuropil regions throughout the brain, with the strongest expression in the hippocampus, the cerebellar cortex, and the olfactory bulb. Immunoelectron microscopy showed that Bassoon immunoreactivity is found in both asymmetric type 1 and symmetric type 2 synapses. Immunopositive asymmetric synapses include mossy fiber boutons and various spine and shaft synapses in the hippocampus and mossy fiber terminals and parallel fiber terminals in the cerebellum. Bassoon-containing symmetric synapses are observed, e.g., between basket and granule cells in the hippocampus, between Golgi cells and granule cells, and between basket cells and Purkinje cells in the cerebellum. Within synaptic terminals, Bassoon appears highly concentrated at sites opposite to postsynaptic densities. In cultured hippocampal neurons, Bassoon was found to colocalize with GABA(A) and glutamate (GluR1) receptors. These data indicate that Bassoon is a component of the presynaptic apparatus of both excitatory glutamatergic and inhibitory GABAergic synapses.",
keywords = "Cerebellum, GABA, Glutamate, Hippocampus, Neurotransmitters, Presynaptic terminal",
author = "Karin Richter and Kristina Langnaese and Kreutz, {Michael R.} and Gisela Olias and Zhai, {Rong G} and Henning Scheich and Garner, {Craig C.} and Gundelfinger, {Eckart D.}",
year = "1999",
month = "6",
day = "7",
doi = "10.1002/(SICI)1096-9861(19990607)408:3<437::AID-CNE9>3.0.CO;2-5",
language = "English",
volume = "408",
pages = "437--448",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Presynaptic cytomatrix protein bassoon is localized at both excitatory and inhibitory synapses of rat brain

AU - Richter, Karin

AU - Langnaese, Kristina

AU - Kreutz, Michael R.

AU - Olias, Gisela

AU - Zhai, Rong G

AU - Scheich, Henning

AU - Garner, Craig C.

AU - Gundelfinger, Eckart D.

PY - 1999/6/7

Y1 - 1999/6/7

N2 - Bassoon is a 420-kDa protein specifically localized at the active zone of presynaptic nerve terminals. It is thought to be involved in the structural organization of the neurotransmitter release site. We studied the distribution of Bassoon transcripts and protein in rat brain and assessed which types of presynaptic terminals contain the protein. As shown by in situ hybridization, Bassoon transcripts are widely distributed in the brain and occur primarily in excitatory neurons. In addition, examples of γ- aminobutyric acid (GABA)-ergic neurons expressing Bassoon are detected. At the light microscopic level, Bassoon immunoreactivity is found in synaptic neuropil regions throughout the brain, with the strongest expression in the hippocampus, the cerebellar cortex, and the olfactory bulb. Immunoelectron microscopy showed that Bassoon immunoreactivity is found in both asymmetric type 1 and symmetric type 2 synapses. Immunopositive asymmetric synapses include mossy fiber boutons and various spine and shaft synapses in the hippocampus and mossy fiber terminals and parallel fiber terminals in the cerebellum. Bassoon-containing symmetric synapses are observed, e.g., between basket and granule cells in the hippocampus, between Golgi cells and granule cells, and between basket cells and Purkinje cells in the cerebellum. Within synaptic terminals, Bassoon appears highly concentrated at sites opposite to postsynaptic densities. In cultured hippocampal neurons, Bassoon was found to colocalize with GABA(A) and glutamate (GluR1) receptors. These data indicate that Bassoon is a component of the presynaptic apparatus of both excitatory glutamatergic and inhibitory GABAergic synapses.

AB - Bassoon is a 420-kDa protein specifically localized at the active zone of presynaptic nerve terminals. It is thought to be involved in the structural organization of the neurotransmitter release site. We studied the distribution of Bassoon transcripts and protein in rat brain and assessed which types of presynaptic terminals contain the protein. As shown by in situ hybridization, Bassoon transcripts are widely distributed in the brain and occur primarily in excitatory neurons. In addition, examples of γ- aminobutyric acid (GABA)-ergic neurons expressing Bassoon are detected. At the light microscopic level, Bassoon immunoreactivity is found in synaptic neuropil regions throughout the brain, with the strongest expression in the hippocampus, the cerebellar cortex, and the olfactory bulb. Immunoelectron microscopy showed that Bassoon immunoreactivity is found in both asymmetric type 1 and symmetric type 2 synapses. Immunopositive asymmetric synapses include mossy fiber boutons and various spine and shaft synapses in the hippocampus and mossy fiber terminals and parallel fiber terminals in the cerebellum. Bassoon-containing symmetric synapses are observed, e.g., between basket and granule cells in the hippocampus, between Golgi cells and granule cells, and between basket cells and Purkinje cells in the cerebellum. Within synaptic terminals, Bassoon appears highly concentrated at sites opposite to postsynaptic densities. In cultured hippocampal neurons, Bassoon was found to colocalize with GABA(A) and glutamate (GluR1) receptors. These data indicate that Bassoon is a component of the presynaptic apparatus of both excitatory glutamatergic and inhibitory GABAergic synapses.

KW - Cerebellum

KW - GABA

KW - Glutamate

KW - Hippocampus

KW - Neurotransmitters

KW - Presynaptic terminal

UR - http://www.scopus.com/inward/record.url?scp=0033532498&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033532498&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9861(19990607)408:3<437::AID-CNE9>3.0.CO;2-5

DO - 10.1002/(SICI)1096-9861(19990607)408:3<437::AID-CNE9>3.0.CO;2-5

M3 - Article

C2 - 10340516

AN - SCOPUS:0033532498

VL - 408

SP - 437

EP - 448

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 3

ER -