Presence of MUC4 in human milk and at the luminal surfaces of blood vessels

Jin Zhang, Aymee Perez, Mohammad Yasin, Pedro Soto, Min Rong, George Theodoropoulos, Coralie A Carothers Carraway, Kermit L. Carraway

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

MUC4 is a heterodimeric membrane mucin, composed of a mucin subunit ASGP-1 (MUC4α) and a transmembrane subunit ASGP-2 (MUC4β), which has been implicated in the protection of epithelial cell surfaces. Surprisingly, development and characterization of a new monoclonal antibody (mAb), called 1G8, against ASGP-2 demonstrated by immunohistochemistry the presence of MUC4 at the luminal surfaces of blood vessels of both normal tissues and tumors. Muc4 was detected with 1G8 and other Muc4 antibodies in blood vessels from humans, rats and mice. This expression of MUC4 in endothelial cells was confirmed by immunoblotting with 1G8 in human umbilical vein endothelial cells (HUVECs), human iliac artery endothelial cells (HIAECs), and human microvascular endothelial cells (HMVECs). MUC4 could be observed on HUVECs grown on either plastic or Matrigel. Finally, MUC4 expression in the three types of endothelial cell lines was confirmed by reverse transcription-polymerase chain reaction (RT-PCR). These results provide, to our knowledge, the first demonstration of a member of the MUC gene family and membrane mucin in blood vessels. As a luminal surface component, the MUC4 is situated to contribute to the non-adhesive luminal surface and to act as an intrinsic protection and survival factor.

Original languageEnglish
Pages (from-to)166-177
Number of pages12
JournalJournal of Cellular Physiology
Volume204
Issue number1
DOIs
StatePublished - Jul 1 2005

Fingerprint

Mucin-4
Endothelial cells
Blood vessels
Human Milk
Blood Vessels
Mucins
Endothelial Cells
Human Umbilical Vein Endothelial Cells
Membranes
Iliac Artery
Immunoblotting
Plastics
Reverse Transcription
Epithelial Cells
Polymerase chain reaction
Immunohistochemistry
Monoclonal Antibodies
Transcription
Cell Line
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Zhang, J., Perez, A., Yasin, M., Soto, P., Rong, M., Theodoropoulos, G., ... Carraway, K. L. (2005). Presence of MUC4 in human milk and at the luminal surfaces of blood vessels. Journal of Cellular Physiology, 204(1), 166-177. https://doi.org/10.1002/jcp.20277

Presence of MUC4 in human milk and at the luminal surfaces of blood vessels. / Zhang, Jin; Perez, Aymee; Yasin, Mohammad; Soto, Pedro; Rong, Min; Theodoropoulos, George; Carraway, Coralie A Carothers; Carraway, Kermit L.

In: Journal of Cellular Physiology, Vol. 204, No. 1, 01.07.2005, p. 166-177.

Research output: Contribution to journalArticle

Zhang, J, Perez, A, Yasin, M, Soto, P, Rong, M, Theodoropoulos, G, Carraway, CAC & Carraway, KL 2005, 'Presence of MUC4 in human milk and at the luminal surfaces of blood vessels', Journal of Cellular Physiology, vol. 204, no. 1, pp. 166-177. https://doi.org/10.1002/jcp.20277
Zhang J, Perez A, Yasin M, Soto P, Rong M, Theodoropoulos G et al. Presence of MUC4 in human milk and at the luminal surfaces of blood vessels. Journal of Cellular Physiology. 2005 Jul 1;204(1):166-177. https://doi.org/10.1002/jcp.20277
Zhang, Jin ; Perez, Aymee ; Yasin, Mohammad ; Soto, Pedro ; Rong, Min ; Theodoropoulos, George ; Carraway, Coralie A Carothers ; Carraway, Kermit L. / Presence of MUC4 in human milk and at the luminal surfaces of blood vessels. In: Journal of Cellular Physiology. 2005 ; Vol. 204, No. 1. pp. 166-177.
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