Presence of autocrine hepatocyte growth factor-Met signaling and its role in proliferation and migration of SNU-484 gastric cancer cell line

Minseon Park, Hyelee Park, Wook Hwan Kim, Hyeseong Cho, Jae Ho Lee

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Autocrine stimulation via coexpression of hepatocyte growth factor (HGF) and its receptor (Met) has been reported in many human sarcomas, but few in carcinomas. In this report, we found that one gastric cancer cell line, SNU-484, among 11 gastric cell lines tested has an autocrine HGF-Met stimulation. RT-PCR, ELISA and scattering assay using MDCK cells revealed that SNU-484 cells secreted a significant amount of active HGF (about 1.25 ± 0.41 ng/24 h/106 cells) into conditioned medium. Resultantly, Met in this cell line was constitutively phosphorylated. Neutralizing antibodies against HGF reduced the tyrosine phosphorylation of Met, resulting in the inhibition of cell proliferation and migration (P < 0.005). To the best of our knowledge, this is the first report on autocrine HGF-Met signaling in a gastric cancer cell line. Our observations with SNU-484 cells suggest that HGF is involved in the development and/or progression of some gastric carcinoma through an autocrine mechanism.

Original languageEnglish (US)
Pages (from-to)213-219
Number of pages7
JournalExperimental and Molecular Medicine
Volume37
Issue number3
DOIs
StatePublished - Jun 30 2005
Externally publishedYes

Keywords

  • Autocrine
  • Gastric cancer
  • Hepatocyte growth factor
  • Met
  • Migration
  • Proliferation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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