Presence of a Na+/H+ exchanger in acidocalcisomes of Leishmania donovani and their alkalization by anti-leishmanial drugs

Anibal E. Vercesi, Claudia O. Rodrigues, Rosana Catisti, Roberto Docampo

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Acidocalcisomes are acidic vacuoles present in trypanosomatids that contain most of the cellular calcium. The data presented here demonstrate that Leishmania donovani acidocalcisomes possess a Na+/H+ exchanger. 3,5- Dibutyl-4-hydroxytoluene, in the concentration range of 0-20 μM, inhibited the Na+/H+ exchanger, and strongly stimulated the activity of the vacuolar H+-ATPase responsible for vacuolar acidification. As occurs with Na+, the cationic anti-leishmanial drugs pentamidine, WR-6026, and chloroquine promoted a fast and extensive alkalization of the L. donovani acidocalcisomes. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)203-206
Number of pages4
JournalFEBS letters
Volume473
Issue number2
DOIs
StatePublished - May 12 2000
Externally publishedYes

Keywords

  • 3,5-Dibutyl-4- hydroxytoluene
  • Acidocalcisome
  • Leishmania donovani
  • Na/H exchanger
  • Volutin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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