Presence of a Na+/H+ exchanger in acidocalcisomes of Leishmania donovani and their alkalization by anti-leishmanial drugs

Anibal E. Vercesi, Claudia D Rodrigues, Rosana Catisti, Roberto Docampo

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Acidocalcisomes are acidic vacuoles present in trypanosomatids that contain most of the cellular calcium. The data presented here demonstrate that Leishmania donovani acidocalcisomes possess a Na+/H+ exchanger. 3,5- Dibutyl-4-hydroxytoluene, in the concentration range of 0-20 μM, inhibited the Na+/H+ exchanger, and strongly stimulated the activity of the vacuolar H+-ATPase responsible for vacuolar acidification. As occurs with Na+, the cationic anti-leishmanial drugs pentamidine, WR-6026, and chloroquine promoted a fast and extensive alkalization of the L. donovani acidocalcisomes. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)203-206
Number of pages4
JournalFEBS Letters
Volume473
Issue number2
DOIs
StatePublished - May 12 2000
Externally publishedYes

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Keywords

  • 3,5-Dibutyl-4- hydroxytoluene
  • Acidocalcisome
  • Leishmania donovani
  • Na/H exchanger
  • Volutin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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