Preponderance of chimerism in ione marrow aspirates when compared to peripheral ilood of kidney recipients receiving ione marrow infusions: as demonstrated by a quantitative sequence specific priming pcr assay

R. Dmccn, M. Harimii, M. Carrano, R. Garcia, T. Karabas, C. Rlcnrdl, D. Tnmpla, H. Famandez, George W Burke, Y. Fanuanal, A. Tafcb, J. Ulur

Research output: Contribution to journalArticle

Abstract

CNmerism, the occurrence of donor Is In long term sold organ recipients has been associated wttbothaoaptanceandreMontfthalrtranspleiMofgans. Our center has isolated bone marrow tram the same cadMrtc kidney donor and Mused SrtOJ per kg on day five and day eleven post amp Malorow UFK50 filinim Kiouppi slwlheiapy, in an attempt to induce a state of tolerance. To test for chimerism Mac crest Bone Marrow Aspirates (BMA) and Peripheral bkxxKPB) samples were taken Iron these recipients from six months to one year post transplant and analyzed by a Sequence Specific Priming (SSP) PCR assay that quanttatos the donor mismatched Class 11DNA from > 5% to 001% or one donor eel in ten thousand recipient cells. Ten Udmy-BMI wen analyzed win a level in their bone marrow of 0.84%(±0.14)ascomparedtottir PBo (0.26%(±OA). Whfcten controls (kidney transplant alone) did not demonstrate any chimerism hi both BMA and PB using this assay. These results demonstrate engraftment of donor ceHs in the BMA of BMI kidney recipients approximaWy 4 x that of their peripheral Mood. Whether this cMnerism represents an acceptance or lack of immune response to these cells by the recipient's Immune system is smi tobedMermined.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996
Externally publishedYes

Fingerprint

Chimerism
chimerism
bone marrow
Assays
Bone
Bone Marrow
kidneys
Kidney
assays
Transplants
Eels
kidney transplant
Immune system
emotions
eel
immune system
Immune System
Iron
immune response
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Preponderance of chimerism in ione marrow aspirates when compared to peripheral ilood of kidney recipients receiving ione marrow infusions : as demonstrated by a quantitative sequence specific priming pcr assay. / Dmccn, R.; Harimii, M.; Carrano, M.; Garcia, R.; Karabas, T.; Rlcnrdl, C.; Tnmpla, D.; Famandez, H.; Burke, George W; Fanuanal, Y.; Tafcb, A.; Ulur, J.

In: FASEB Journal, Vol. 10, No. 6, 01.12.1996.

Research output: Contribution to journalArticle

Dmccn, R. ; Harimii, M. ; Carrano, M. ; Garcia, R. ; Karabas, T. ; Rlcnrdl, C. ; Tnmpla, D. ; Famandez, H. ; Burke, George W ; Fanuanal, Y. ; Tafcb, A. ; Ulur, J. / Preponderance of chimerism in ione marrow aspirates when compared to peripheral ilood of kidney recipients receiving ione marrow infusions : as demonstrated by a quantitative sequence specific priming pcr assay. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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abstract = "CNmerism, the occurrence of donor Is In long term sold organ recipients has been associated wttbothaoaptanceandreMontfthalrtranspleiMofgans. Our center has isolated bone marrow tram the same cadMrtc kidney donor and Mused SrtOJ per kg on day five and day eleven post amp Malorow UFK50 filinim Kiouppi slwlheiapy, in an attempt to induce a state of tolerance. To test for chimerism Mac crest Bone Marrow Aspirates (BMA) and Peripheral bkxxKPB) samples were taken Iron these recipients from six months to one year post transplant and analyzed by a Sequence Specific Priming (SSP) PCR assay that quanttatos the donor mismatched Class 11DNA from > 5{\%} to 001{\%} or one donor eel in ten thousand recipient cells. Ten Udmy-BMI wen analyzed win a level in their bone marrow of 0.84{\%}(±0.14)ascomparedtottir PBo (0.26{\%}(±OA). Whfcten controls (kidney transplant alone) did not demonstrate any chimerism hi both BMA and PB using this assay. These results demonstrate engraftment of donor ceHs in the BMA of BMI kidney recipients approximaWy 4 x that of their peripheral Mood. Whether this cMnerism represents an acceptance or lack of immune response to these cells by the recipient's Immune system is smi tobedMermined.",
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AU - Harimii, M.

AU - Carrano, M.

AU - Garcia, R.

AU - Karabas, T.

AU - Rlcnrdl, C.

AU - Tnmpla, D.

AU - Famandez, H.

AU - Burke, George W

AU - Fanuanal, Y.

AU - Tafcb, A.

AU - Ulur, J.

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AB - CNmerism, the occurrence of donor Is In long term sold organ recipients has been associated wttbothaoaptanceandreMontfthalrtranspleiMofgans. Our center has isolated bone marrow tram the same cadMrtc kidney donor and Mused SrtOJ per kg on day five and day eleven post amp Malorow UFK50 filinim Kiouppi slwlheiapy, in an attempt to induce a state of tolerance. To test for chimerism Mac crest Bone Marrow Aspirates (BMA) and Peripheral bkxxKPB) samples were taken Iron these recipients from six months to one year post transplant and analyzed by a Sequence Specific Priming (SSP) PCR assay that quanttatos the donor mismatched Class 11DNA from > 5% to 001% or one donor eel in ten thousand recipient cells. Ten Udmy-BMI wen analyzed win a level in their bone marrow of 0.84%(±0.14)ascomparedtottir PBo (0.26%(±OA). Whfcten controls (kidney transplant alone) did not demonstrate any chimerism hi both BMA and PB using this assay. These results demonstrate engraftment of donor ceHs in the BMA of BMI kidney recipients approximaWy 4 x that of their peripheral Mood. Whether this cMnerism represents an acceptance or lack of immune response to these cells by the recipient's Immune system is smi tobedMermined.

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