Preparation of synthetic tRNA precursors with tRNA nuc1eotidy1transferase

Murray P. Deutscher, Ranajit K. Ghosh

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Rabbit liver tRNA nucleotidyltransferase can be used to substitute nucleotides within the -C-C-A sequence of tRNA or to add nucleotides following this sequence. These anomolous reactions of the enzyme have been used to prepare radioactively-labeled synthetic tRNA precursors which mimic the structure of the natural precursors. Under appropriate conditions synthetic precursors of defined structure can be made. In this paper we describe the synthesis of tRNA-C-[14C]U and tRNA-C-C-A-[14C]-C, which are representative of tRNA precursors containing altered residues within the -C-C-A sequence or with extra residues following the normal 3′terminus. A variety of other possible precursors can also be prepared. These synthetic tRNA precursors have already proved useful for isolation of possible tRNA processing nucleases.

Original languageEnglish (US)
Pages (from-to)3821-3830
Number of pages10
JournalNucleic acids research
Volume5
Issue number10
DOIs
StatePublished - Oct 1 1978

ASJC Scopus subject areas

  • Genetics

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