Prenatal sexing and detection of ZFY gene sequences in sex chromosome disorders by polymerase chain reaction

R. Sasi, Yao-Shan Fan, C. C. Lin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Specific amplification of a DNA segment of the human ZFY gene by polymerase chain reaction (PCR) was carried out for detection of Y-chromosome specific sequences. When male DNA was used as template for amplification, a single and discrete 530 bp ethidium bromide staining band was observed in agarose gel. Female DNA produced no band. This technique was successfully used in prenatal sexing and for detecting ZFY DNA sequences in five XX males, an XY female, and a female patient with Turner syndrome having mosaicism involving a minute marker chromosome.

Original languageEnglish
Pages (from-to)193-196
Number of pages4
JournalJournal of Clinical Laboratory Analysis
Volume5
Issue number3
StatePublished - Jun 6 1991
Externally publishedYes

Fingerprint

Sex Chromosome Disorders
Polymerase chain reaction
Genes
Chromosomes
Polymerase Chain Reaction
Amplification
DNA
Turner Syndrome
Mosaicism
Ethidium
Y Chromosome
DNA sequences
Genetic Markers
Sepharose
Gels
Staining and Labeling
Sex Chromosomes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Prenatal sexing and detection of ZFY gene sequences in sex chromosome disorders by polymerase chain reaction. / Sasi, R.; Fan, Yao-Shan; Lin, C. C.

In: Journal of Clinical Laboratory Analysis, Vol. 5, No. 3, 06.06.1991, p. 193-196.

Research output: Contribution to journalArticle

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