Abstract
Specific amplification of a DNA segment of the human ZFY gene by polymerase chain reaction (PCR) was carried out for detection of Y-chromosome specific sequences. When male DNA was used as template for amplification, a single and discrete 530 bp ethidium bromide staining band was observed in agarose gel. Female DNA produced no band. This technique was successfully used in prenatal sexing and for detecting ZFY DNA sequences in five XX males, an XY female, and a female patient with Turner syndrome having mosaicism involving a minute marker chromosome.
| Original language | English |
|---|---|
| Pages (from-to) | 193-196 |
| Number of pages | 4 |
| Journal | Journal of Clinical Laboratory Analysis |
| Volume | 5 |
| Issue number | 3 |
| State | Published - Jun 6 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Prenatal sexing and detection of ZFY gene sequences in sex chromosome disorders by polymerase chain reaction. / Sasi, R.; Fan, Yao-Shan; Lin, C. C.
In: Journal of Clinical Laboratory Analysis, Vol. 5, No. 3, 06.06.1991, p. 193-196.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prenatal sexing and detection of ZFY gene sequences in sex chromosome disorders by polymerase chain reaction
AU - Sasi, R.
AU - Fan, Yao-Shan
AU - Lin, C. C.
PY - 1991/6/6
Y1 - 1991/6/6
N2 - Specific amplification of a DNA segment of the human ZFY gene by polymerase chain reaction (PCR) was carried out for detection of Y-chromosome specific sequences. When male DNA was used as template for amplification, a single and discrete 530 bp ethidium bromide staining band was observed in agarose gel. Female DNA produced no band. This technique was successfully used in prenatal sexing and for detecting ZFY DNA sequences in five XX males, an XY female, and a female patient with Turner syndrome having mosaicism involving a minute marker chromosome.
AB - Specific amplification of a DNA segment of the human ZFY gene by polymerase chain reaction (PCR) was carried out for detection of Y-chromosome specific sequences. When male DNA was used as template for amplification, a single and discrete 530 bp ethidium bromide staining band was observed in agarose gel. Female DNA produced no band. This technique was successfully used in prenatal sexing and for detecting ZFY DNA sequences in five XX males, an XY female, and a female patient with Turner syndrome having mosaicism involving a minute marker chromosome.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025803857&partnerID=8YFLogxK
M3 - Article
C2 - 2061743
AN - SCOPUS:0025803857
VL - 5
SP - 193
EP - 196
JO - Journal of Clinical Laboratory Analysis
JF - Journal of Clinical Laboratory Analysis
SN - 0887-8013
IS - 3
ER -