Preliminary quantitative proteomic characterization of glaucomatous rat retinal ganglion cells

John W. Crabb, Xianglin Yuan, Galina Dvoriantchikova, Dmitry Ivanov, John S. Crabb, Valery I. Shestopalov

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Quantitative proteomic analysis was pursued of retinal ganglion cells (RGCs) from rats with unilateral experimental glaucoma. RGCs were isolated from 22 animals by immunopanning after 8 weeks of sustained elevated intraocular pressure. Proteins were quantified by LC MS/MS iTRAQ technology. Of the 268 proteins quantified, ∼8% appeared elevated and ∼13% decreased in glaucomatous RGCs. Voltage-dependent anion channel protein 2, aldose reductase, and ubiquitin were among the significantly elevated proteins while prothymosin was among the significantly decreased. The results demonstrate the feasibility of identifying global proteomic differences in protein expression between purified glaucomatous and control in vivo RGCs.

Original languageEnglish (US)
Pages (from-to)107-110
Number of pages4
JournalExperimental Eye Research
Volume91
Issue number1
DOIs
StatePublished - Jul 2010

Keywords

  • Experimental glaucoma
  • ITRAQ technology
  • Mass spectrometry
  • Quantitative proteomics
  • Retinal ganglion cells

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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